AnaSpec is pleased to announce the inclusion of two new dyes in its AnaTag^™ protein labeling kit collection - HiLyte Fluor^™ 594 (Ex/Em=593/616 nm) and HiLyte Fluor^™ 680 (Ex/Em=678/699 nm). Developed by AnaSpec, HiLyte Fluor^™ dyes are a series of dyes that spans the whole visible spectrum (Figure 1).

AnaTag™ HiLyte Fluor™ Protein Labeling kits provide a convenient way to label proteins using the succinimidyl ester (SE) reactive form of the HiLyte Fluor™ dyes. The succinimidyl ester shows good reactivity and selectivity with aliphatic amines of the protein and forms a carboxamide bond, which is identical to, and is as stable as the natural peptide bond (Figure 2). HiLyte Fluor™‑protein conjugates can sustain treatments during immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications without hydrolysis. The kits can be used for microscale (3 x 200 ug) and larger scale (3 x 5 mg) labeling of proteins, such as antibodies.

• Fast Labeling time - as short as 30 minutes
• All essential components for conjugation reaction and purification of dye-protein conjugates
• Available in two convenient reaction sizes:
Microscale labeling kit: up to 200 ug protein x 3 labelings or Larger scale labeling kit:
up to 5 mg protein x 3 reactions
• Stable dye-protein conjugate products
• Industry-standard amine-reactive chemistry

Figure 1. Absorbance and Fluorescence of HiLyte Fluor™ 488 (1), HiLyte Fluor™ 488 (1), HiLyte Fluor™ 555 (2), HiLyte Fluor™ 594 (3), HiLyte Fluor™ 647 (4), HiLyte Fluor™ 680 (5), and HiLyte Fluor™ 750 (6).

Figure 2. The succinimidyl ester group of fluorophore reacts with amine groups on the protein to form a stable carboxamide bond.

Figure 4. Alpha tubulin in 3T3 cells probed with mouse anti -alpha tubulin antibody, visualized with HiLyte 594 conjugated goat anti-mouse IgG. Nuclei stained with DAPI (cat# 83210).

Related Products:

Entire Line of AnaTag™ Protein Labeling Kits

HiLyte Fluor^™-labeled Secondary Antibodies

HiLyte Fluor^™-labeled Streptavidins

HiLyte Fluor^™ dyes

Custom labeling service available – contact service@anaspec.com to inquire.
AnaSpec is pleased to introduce the AnaTag^™ HiLyte Fluor^™ 594 and 680 Protein Labeling Kits.