AnaSpec is pleased to be the industry’s first provider of
nonmuscle myosin class II heavy chain (NMHC) antibodies.
Nonmuscle myosin class II proteins belong to the superfamily
of actin-based motor proteins. They form hexamer structures composed of two
~200kDa heavy chains (NMHC II) and two pairs of 20kDa and 17kDa light chains
(MLC20 and MLC17) (1,2). Nonmuscle myosins are classified according to the heavy
chain isoforms; light chains are conserved. Three different isoforms that share
64-80% amino acid sequence identity have been detected in mammals: NMHC II-A,
NMHC II-B, and NMHC II-C. Although NMHC II molecules have now been found in
muscle cells as well, the name “nonmuscle myosin” was already widespread and
is in use today. NMHC II-A is most abundant compared to other NMHC isoforms
in human lung, spleen, thymus, uterus, and colon.1 Single amino
acid mutation in MNMH II-A results in kidney and platelet defects in humans.1
In addition, recent study indicates that replacement of NMHC II-B with NMHC
II-A rescues brain defect in mice.3
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|Western blots: HT-29 cell lysate was probed with 1:200
dilution of rabbit anti-NMHC II antibodies: NMHC (CT), cat# 55308 (panel
A), NMHC II-B (CT-1), cat# 55309 (B), NMHC II-B (CT-2), cat# 55310 (C) and
NMHC II-C (CT), cat# 55312 (D).
1. Golomb E. et al. J. Biol. Chem.
279, 2800 (2004).
2. Conti MA. et al. J. of Cell Sci.
121, 11 (2007).
3. Bao J.
et al. J. Biol. Chem. 282, 22102 (2007).