AnaSpec is pleased to announce the newest additions to its expanding line of keratin antibodies. Backed by AnaSpec’s antibody guarantee*, these type I acidic keratin antibodies (keratin –10, -13 and -14) have been tested in applications such as western blots or IHC.

Intermediate filaments (IFs), together with actin microfilaments and tubulin microtubules, comprise the three major cytoskeletal networks that are found in most eukaryotic cells.¹ Keratins, which are the largest IF protein subgroup, can be divided into the acidic type I (K9-K20) and basic type II (K1-K8) Keratins.² Typically, epithelial cells express at least one of each type, and the proteins exist in the cells as noncovalent obligate heteropolymers. Keratin networks are highly dynamic and reorganized during cell differentiation, mitosis and apoptosis, and also play important cytoprotective and structural support roles for the cell.^3,4

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Figure 1. Western blots of anti-keratin-10, 13 and 14. Data courtesy of Dr. Bishr Omary at University of Michigan.

Figure 2. IHCs of FFPE human breast tissue sections probed with anti-keratin-14 (IN), Cat# 55080 at 1:150 dilution. Antigen retrieval was accomplished by using 0.01% pepsin diluted in HCl, pH 2, incubated for 30 min at 37 in a humidified chamber. Secondary antibody used was donkey anti-rabbit labeled with Alexa-488 (Invitrogen), and nuclei were stained with DAPI. Images courtesy of Dr. Andra Frost, Univ. of Alabama at Birmingham.

For a full listing of all our keratin antibodies, please click here.

Related Products:

Anti-actin (labeled or non-labeled)

References:

1. Ku, NO. et al. Am J. Physiol. 277, G1108 (1999).
2. Schweizer, J. et al. J. Cell Biol. 174, 169 (2006).
3. Omary, MB. et al. Trends Biochem. Sci. 31, 383 (2006).
4. Omary, MB. et al. N. Engl. J. Med. 351, 2087 (2004).

* About AnaSpec Antibodies
• Our catalog antibodies are guaranteed to work for applications stated on our data sheet. If our catalog antibody does not work for these applications, we will apply a 100% credit towards your next purchase.
• Guarantee does not apply to applications not listed as "recommended" on the data sheet.
• We are always looking to expand our range of guaranteed applications. Researchers who demonstrate the effectiveness of AnaSpec antibodies in non-guaranteed applications (not listed or listed as "recommended") are eligible for a 50% credit of the list price towards your next purchase of one vial of AnaSpec catalog antibodies. To receive your credit, simply e-mail your data to service@anaspec.com. One vial per catalog antibody only.