Calcein, AM – Live Cell Staining


Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. Non-fluorescent Calcein, AM in live cells is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein (Figure 1), and is retained in the cytoplasm of live cells. The excitation and emission wavelengths of calcein are 495 nm and 516 nm, respectively (Figure 2). Figure 3 shows an image of Hela cells stained with AnaSpec’s Calcein AM fluorescent dye. Amongst other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein, AM is the most suitable indicator for staining viable cells due to its low cytotoxicity. Calcein does not affect any cellular functions such as proliferation or chemotaxis of lymophocyte.


Figure 1. Schematic diagram of viable cell staining


Figure 2. Normalized absorption and emission spectra of calcein in PBS buffer (pH 8.5).


Figure 3. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye.

AnaSpec is pleased to offer the SensoLyte® Calcein Cell Viability Assay Kit. This kit provides ample reagents (dye and assay buffer) for 1000 assays in a 96-well plate format.

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Product

Size

Catalog #

Calcein, AM

1 mg

89201

Calcein, AM *UltraPure Grade*

1 mg

89202

Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO

200 µL

89203

Calcein; AM *UltraPure Grade* *Small Package*

50 µg x 20

89204

SensoLyte™ Calcein Cell Viability Assay Kit *Fluorimetric* NEW

1000 assays

72126

AnaSpec’s Calcein, AM, a green fluorescent cell viability indicator, is an excellent quality, cost-effective solution for live cell staining.