is a cell-permeant and non-fluorescent compound that is widely used for determining
cell viability. Non-fluorescent Calcein, AM in live cells is hydrolyzed by intracellular
esterases into the strongly green fluorescent anion calcein (Figure 1), and
is retained in the cytoplasm of live cells. The excitation and emission wavelengths
of calcein are 495 nm and 516 nm, respectively (Figure 2). Figure 3 shows an
image of Hela cells stained with AnaSpec’s Calcein AM fluorescent dye. Amongst
other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein,
AM is the most suitable indicator for staining viable cells due to its
low cytotoxicity. Calcein does not affect any cellular functions such as proliferation
or chemotaxis of lymophocyte.
Figure 1. Schematic diagram of viable cell staining
Figure 2. Normalized absorption and emission spectra of calcein in PBS buffer
Figure 3. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye.
pleased to offer the SensoLyte®
Calcein Cell Viability Assay Kit. This kit provides ample reagents
(dye and assay buffer) for 1000 assays in a 96-well plate format.
here to request more info
Calcein, AM, a green fluorescent cell viability indicator, is an excellent quality,
cost-effective solution for live cell staining.