AnaSpec, the provider of
the world’s largest selection of β-amyloid peptides and related products
for Alzheimer’s disease (AD) research, is pleased to showcase its β-amyloid
peptides labeled with biotin (N or C-terminal biotin labels) as well as N-terminal
biotin labeled β-amyloid peptides with a fluorescein label on the C-terminus.
These peptides have been used in different applications. Nelson,
TJ and Alkon, DL. used biotin-LC-β-amyloid-(1–42)
in Aβ-proteins binding studies.1 Aβ-proteins complex were
precipitated with avidin-agarose and western blots run. A paper by Bohrmann,
B. et al. reported the use of biotin-Aβ (1-40) or
biotin-Aβ (1-42) for their Aβ polymerization studies. 2
Biotin-beta-amyloid (1-40) or biotin-beta-amyloid (1-42) peptides
were incubated in Aβ (1-40) or Aβ (1-42) coated plates,
and streptavidin-peroxidase added. Color development was done using tetramethyl-benzidin,
a chromogenic substrate for peroxidase. In the same paper,2 biotin-beta-amyloid
(1-40) incorporation into preformed Aβ (1-42) fibrils was reported.
Biotin-Aβ (1-40) was added to a low concentration of Aβ (1-42) fibrils
and stained with anti-biotin Ig labeled with colloidal gold and negatively
stained with uranyl acetate. A paper by Liu, Y. et al shows the use of biotinylated
fibrillar Aβ (1-42) in electron microscopy.3
Using what they called “FAβB,” fluorescein beta-amyloid (1-40)-Lys(LC-biotin),
Leissring, MA. et al. performed proteolysis measurement using fluorescence polarization
Table 1. A listing of biotin labeled beta-amyloid peptides, green shading
denotes beta-amyloid peptides with an amidated C-terminus.
Table 2. A listing of N-terminal labeled biotin and C-terminal fluorescein (5-FAM)
beta-amyloid (1-40) and beta-amyloid (1-42).
Custom peptide synthesis is also available, please contact
Peptides, full listing
β-Amyloid Peptides – heavy isotope labeled peptides
Peptides (1-44, 1-45, 1-46, 1-47, 1-48 & 1-49)
Alpha-Secretase Assay Kit
Antibodies – phospho and non-phosphorylated
fluorescent b-Amyloid (1-40) & (1-42) Sampler Kits
TJ. and DL. Alkon, J. Biol. Chem. 282, 31238 (2007).
B. et al. J. Biol. Chem. 274, 15990 (1999).
Y. et al. Brain 128, 1778 (2005).
MA. et al. J. Biol. Chem. 278, 37314 (2003).