AnaSpec, the provider of
the world’s largest selection of β-amyloid peptides and related products
for Alzheimer’s disease (AD) research, is pleased to showcase its β-amyloid
peptides labeled with biotin (N or C-terminal biotin labels) as well as N-terminal
biotin labeled β-amyloid peptides with a fluorescein label on the C-terminus.
These peptides have been used in different applications. Nelson,
TJ and Alkon, DL. used biotin-LC-β-amyloid-(1–42)
in Aβ-proteins binding studies.1 Aβ-proteins complex were
precipitated with avidin-agarose and western blots run. A paper by Bohrmann,
B. et al. reported the use of biotin-Aβ (1-40) or
biotin-Aβ (1-42) for their Aβ polymerization studies. 2
Biotin-beta-amyloid (1-40) or biotin-beta-amyloid (1-42) peptides
were incubated in Aβ (1-40) or Aβ (1-42) coated plates,
and streptavidin-peroxidase added. Color development was done using tetramethyl-benzidin,
a chromogenic substrate for peroxidase. In the same paper,2 biotin-beta-amyloid
(1-40) incorporation into preformed Aβ (1-42) fibrils was reported.
Biotin-Aβ (1-40) was added to a low concentration of Aβ (1-42) fibrils
and stained with anti-biotin Ig labeled with colloidal gold and negatively
stained with uranyl acetate. A paper by Liu, Y. et al shows the use of biotinylated
fibrillar Aβ (1-42) in electron microscopy.3
Using what they called “FAβB,” fluorescein beta-amyloid (1-40)-Lys(LC-biotin),
Leissring, MA. et al. performed proteolysis measurement using fluorescence polarization
(FP) technique.4
Table 1. A listing of biotin labeled beta-amyloid peptides, green shading
denotes beta-amyloid peptides with an amidated C-terminus.
Table 2. A listing of N-terminal labeled biotin and C-terminal fluorescein (5-FAM)
beta-amyloid (1-40) and beta-amyloid (1-42).
Custom peptide synthesis is also available, please contact
service@anaspec.com
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References:
- Nelson,
TJ. and DL. Alkon, J. Biol. Chem. 282, 31238 (2007).
- Bohrmann,
B. et al. J. Biol. Chem. 274, 15990 (1999).
- Liu,
Y. et al. Brain 128, 1778 (2005).
- Leissring,
MA. et al. J. Biol. Chem. 278, 37314 (2003).
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