In response to the growing needs of the research community for zebrafish specific antibodies, peptides and other products, AnaSpec has launched the exciting new Z-Fish™ series of antibodies. Z-Fish™ antibodies are raised strictly from peptide antigens derived from zebrafish protein sequences, i.e. 100% zebrafish sequence homology. Leveraging AnaSpec’s expertise in peptide technology, the antigens used in raising these antibodies include non-phosphorylated and phosphorylated peptides. Z-Fish™ antibodies are provided in a 150 µl size, which is optimal for 15 mini gels.

Zebrafish has been used as a developmental model in elucidating the role of MMPs during embryogenesis/development. Zhang, J. et al. isolated and characterized zMMP-2 (which shows >80% identity and >90% similarity to its human counterpart);¹ Yoong, et al, zMMP-9; ² and Hildegass, JM. et al. zMMP-13 (52% identity with human or mouse);³ among others. AnaSpec is pleased to introduce a line of Z-Fish™ antibodies for MMP-2, 9, 11, 13, 14, 17 and 24. These antibodies were raised from sequences derived from the hinge region, internal or C-terminal sequence. They have been verified in ELISA and western blots using zebrafish lysates (see western blots below).

If you have any suggestions for additional Z-Fish™ antibodies, please contact us at ab@anaspec.com

References:

1. Zhang, J. et al. Dev. Genes Evol. 213, 456 (2004).
2. Yoong, S. et al. Genes Expr. Patterns 7, 39 (2007).
3. Hillegass, JM. et al. Toxicol. Sci. 100, 168 (2007).