Fluorescent Lipophilic Tracers - Wide Selection


Carbocyanine dyes are widely used as lipophilic tracers to label cells, organelles, liposomes, viruses and lipoproteins. These dyes can be used in a wide variety of application for long-term tracing of cells, including cell transplantation, migration, adhesion and fusion studies.

The most popular lipophilic tracers, DiO (DiOC18(3)), DiI (DiIC18(3)), DiD (DiIC18(5)) and DiR (DiIC18(7)) have extremely high extinction coefficients, environmental dependent fluorescence and short excited-state lifetimes. They are weakly fluorescent in aqueous phase, but highly fluorescent and photostable when incorporated into membranes or bound to lipophilic biomolecules. These optical characteristics make them ideal for staining the cytoplasmic membranes of cells. Once applied to cells, these dyes diffuse laterally within the plasma membrane, resulting in staining of the entire cell.

DiO, DiI, DiD and DiR exhibit distinct green, orange, red and infrared fluorescence, respectively (See Figure 1), thus facilitating multicolor imaging and fluorescence flow cytometric analysis of live cells.


Figure 1. Normalized fluorescence spectra of DiO, DiI, DiD and DiR in methanol
.

To improve on the slow diffusion rate of lipophilic tracers from the point of their application to the terminus of a neuron, AnaSpec offers lipophilic probes with unsaturated alkyl chains, including SPEEDY DiO, SPEEDY DiA, SPEEDY DiI and Δ 9-DiI, to accelerate the diffusion processes.

Product Size Catalog #
δ9 - DiI
[1,1' - Dioleyl - 3,3,3',3' - tetramethylindocarbocyanine methanesulfonate]
25 mg 84906
DiA
[4 - (4 - (Dihexadecylamino)styryl) - N - methylpyridinium iodide]
25 mg 84710
DiD
[1,1' - Dioctadecyl - 3,3,3',3' - tetramethylindodicarbocyanine iodide] *Oil*
25 mg 84903
DiD
[1,1' - Dioctadecyl - 3,3,3',3' - tetramethylindodicarbocyanine, 4 - chlorobenzenesulfonate salt] *Solid*
10 mg 84920
DIDS
[4,4' - Diisothiocyanatostilbene - 2,2' - disulfonic acid, disodium salt]
100 mg 84904
DiI
[1,1' - Dioctadecyl - 3,3,3',3' - tetramethylindocarbocyanine iodide]
100 mg 84711
DiIC1(5)
[1,1',3,3,3',3' - Hexamethylindodicarbocyanine iodide]
100 mg 84709
DiIC12(3)
[1,1' - Didodecyl - 3,3,3',3' - tetramethylindocarbocyanine iodade]
100 mg 84902
DiIC16(3)
[1,1' - Dihexadecyl - 3,3,3',3' - tetramethylindocarbocyanine iodade]
100 mg 84905
DiO
[3,3' - Dioctadecyloxacarbocyanine iodide]
25 mg 84712
DiOC16(3)
[3,3' - Dihexadecyloxacarbocyanine iodade]
25 mg 84708
DiOC2(3)
[3,3' - Diethyloxacarbocyanine iodide]
100 mg 84706
DiOC5(3)
[3,3' - Dipentyloxacarbocyanine iodide]
100 mg 84714
DiOC6(3)
[3,3' - Dihexyloxacarbocyanine iodide]
100 mg 84715
DiOC7(3)
[3,3' - Diheptyloxacarbocyanine iodide]
100 mg 84707
DiR
[1,1' - Dioctadecyl - 3,3,3',3' - tetramethylindotricarbocyanine iodide]
10 mg 84922
DiSBAC2(3)
[Bis - (1,3 - diethylthiobarbituric acid)trimethine oxonol]
25 mg 84702
DiSC3(5)
[3,3' - Dipropylthiadicarbocyanine iodide]
100 mg 84923
5-Dodecanoylaminofluorescein
100 mg 88200
Laurdan
[6 - Dodecanoyl - 2 - dimethylaminonaphthalene]
25 mg 88201
Prodan
[6 - Propionyl - 2 - dimethylaminonaphthalene]
25 mg 88212
SPEEDY™ DiA
[4 - (4 - (Dilinoleylamino)styryl) - N - methylpyridinium iodide] *Oil*
5 mg 84732
SPEEDY™ DiA
[4 - (4 - (Dilinoleylamino)styryl) - N - methylpyridinium 4 - chlorobenzenesulfonate] *Solid*
5 mg 84733
SPEEDY™ DiI
[1,1' - Dilinoleyl - 3,3,3',3' - tetramethylindocarbocyanine perchlorate] *Oil*
5 mg 84734
SPEEDY™ DiI
[1,1' - Dilinoleyl - 3,3,3',3' - tetramethylindocarbocyanine 4 - chlorobenzenesulfonate] *Solid*
5 mg 84735
SPEEDY™ DiO
[3,3' - Dilinoleyloxacarbocyanine perchlorate] *Solid*
5 mg 84912

Click here for a full listing of AnaSpec’s dye products. For custom syntheses of dyes, please contact service@anaspec.com.

AnaSpec is pleased to highlight our wide selection of fluorescent liphophilic tracers, which are of the highest quality, and are cost-effective.