MOG Proteins, Peptides, Antibodies & Kits


Myelin Oligodendrocyte Glycoprotein (MOG), a 26- to 28-kDa protein, is a member of the immunoglobulin superfamily. It is expressed exclusively in the central nervous system (CNS), specifically on the surface of myelinating oligodendrocytes and external lamellae of myelin sheath. Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys (1-6).

AnaSpec is pleased to be the first in the industry to provide a whole suite of reagents and assay kits for the advancement of MS research. This includes MOG proteins, peptides, antibodies and assay kits.

MOG Proteins

These proteins, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag were expressed in E. coli and purified from urea denatured bacterial lysate. The molecular weight found in all 3 recombinant proteins was 14.23 kDa. These proteins were shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for rat rMOG).

Recombinant MOG Protein

Size

Catalog #

Recombinant human MOG NEW

100 µg

500 µg

1000 µg

55158-100

55158-500

55158-1000

Recombinant mouse MOG NEW

100 µg

500 µg

1000 µg

55150-100

55150-500

55150-1000

Recombinant rat MOG NEW

100 µg

500 µg

1000 µg

55152-100

55152-500

55152-1000



Figure 1. Western blots of MOG recombinant proteins for human (A), mouse (B) and rat (C).

MOG (35-55) and other MOG Peptide Fragments

AnaSpec’s MOG (35-55) peptide from mouse and rat sequence (cat# 60130) was shown to induce anti-MOG (35-55) autoantibody production in C57BL/6 mice at 100% incidence (10/10); providing quantitative data that this peptide is suitable for in vivo anti-MOG (35-55) autoantibody study. A cysteine-containing MOG (35-55) peptide induced polyclonal anti-MOG (35-55) antibody production in rabbits with high specificity, high affinity, and high titer indicating its high purity, as well as suitability for use in peptide-coated ELISA and Western blot. This mouse/rat MOG (35-55) peptide was used as a coating peptide to develop the industry’s first anti-MOG (35-55) ELISA kit for EAE study. The detection limit of the kit was determined to reach as low as 50 pg of anti-MOG (35-55) autoantibody in serum or cerebrospinal fluid. Lot to lot variability of the MOG (35-55) was found to be minimal.

MOG Antibodies

Two rabbit polyclonal antibodies towards the MOG extracellular domain are available; one raised against the human recombinant protein (cat# 55158) and the other against the mouse recombinant protein (cat# 55150). These Protein A affinity purified antibodies recognized a 27kDa band.

Product

Size

Cat #

"Anti-MOG, human" NEW
"Host: rabbit polyclonal; Species Reactivity: human, mouse, rat; Application: WB"

50 µg

55161

"Anti-MOG, mouse" NEW
"Host: rabbit polyclonal; Species Reactivity: mouse, human; Application: WB"

50 µg

55160

SensoLyte® Anti-MOG IgG ELISA Assay Kits

The SensoLyte Anti-Human MOG (1-125) IgG Quantitation ELISA Kit, SensoLyte® Anti-Mouse MOG (1-125) and SensoLyte® Anti-Rat MOG (1-125) IgG ELISA assay kits are useful for determining the amount of anti-human MOG (1-125), anti-mouse MOG (1-125) and anti-rat MOG (1-125) antibodies, respectively. These kits, as well as the recombinant MOG (1-125) proteins are examples of innovative products from AnaSpec.

SensoLyte® Anti-Human MOG (1-125) Assay

SensoLyte® Anti-Mouse MOG (1-125) Assay

SensoLyte® Anti-Rat MOG (1-125) Assay

Specificity

Human

Mouse

Rat

Format

Human rMOG (1-125) precoated 12-strip plate

Mouse rMOG (1-125) precoated 12-strip plate

Rat rMOG (1-125) precoated 12-strip plate

Standard(s) Included

Mouse & Rat anti-human MOG (1-125) IgG standards

Anti-mouse MOG (1-125) IgG standard

Anti-rat MOG (1-125) IgG standard

Sensitivity

Detects as low as 1 ng anti-human MOG (1-125)

Detects as low as 1 ng anti-mouse MOG (1-125)

Detects as low as 1 ng anti-rat MOG (1-125)

Assay Time

2-3 hours assay time at room temperature

Sample Concentration

0.5-1 μl of serum or cerebrospinal fluid

Read-out

Colorimetric read-out at 450 nm absorbance

Broad Dynamic Range

8-500 ng IgG/ml serum (depending on colorimetric developing time)



The SensoLyte® Anti-MOG (35-55) IgG Quantitative ELISA kits are optimized to detect anti-MOG (35-55) mouse/rat IgG and IgG subtypes. Using strips pre-coated with MOG (35-55) peptide and pre-blocked with BSA, the amount of anti-MOG IgG subtypes in serum or cerebrospinal fluid can be determined by ELISA and the absorbance read at 450 nm.

SensoLyte® Anti - MOG(35 - 55) IgG Quantitative ELISA Kit (mouse/rat)

54465

SensoLyte® Anti - MOG(35 - 55) IgG Subtype Quantitative ELISA Kit (mouse/rat)

54471

SensoLyte® Anti - MOG(35 - 55) IgG1 Quantitative ELISA Kit (mouse/rat)

54466

SensoLyte® Anti - MOG(35 - 55) IgG2a Quantitative ELISA Kit (mouse/rat)

54467

SensoLyte® Anti - MOG(35 - 55) IgG2b Quantitative ELISA Kit (mouse/rat)

54468

SensoLyte® Anti - MOG(35 - 55) IgG2c Quantitative ELISA Kit (mouse/rat)

54469

SensoLyte® Anti - MOG(35 - 55) IgG3 Quantitative ELISA Kit (mouse/rat)

54470

Related Products:

MBP Peptides
PLP Peptides
Anti-PLPs
Anti-MBP

References:

1. Linares, D. et al. Protein Expression and Purif. 34, 249 (2004).
2. Bettadapura, J. et al. J. Neurochem. 70, 1593 (1998).
3. Oliver, AR. et al J. Immunol. 171, 462 (2003).
4. Von Budingen, H-C. et al. J. Clin. Immunol. 21, 155 (2001).
5. Lyons, J-A. et.al. Eur. J. Immunol. 29, 3432 (1999).
6. Von Budingen, H-C. et.al. Eur. J. Immunol. 34, 2072 (2003 ).