AnaSpec is pleased to introduce the newest member of the HiLyte Fluor™ family
- HiLyte Fluor™ 405 (Ex/Em=404/428 nm), a bright blue fluorescent probe.

HiLyte Fluor™ dyes are a series of superior fluorescent labeling dyes that span the full visible spectrum. They are optimized for maximum brightness when conjugated with macromolecules.

Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, HiLyte Fluor™ 405 allows for easy conjugation to biopolymers. HiLyte Fluor™ 405 has spectral characteristics similar to those of Alexa Fluor™ 405 (Invitrogen) and DyLight Fluor™ 405 (Pierce) dyes with excitation and emission wavelength at 404/428 nm in PBS buffer (pH 7.4) (see spectra below). Conjugates of HiLyte Fluor™ 405 are well excited by the 407 nm spectral line of the Krypton laser and the 408 nm line of the violet laser diode.

Table 1. HiLyte Fluor™ 405 is available in different reactive forms.

HiLyte Fluor™ 405 Labeled Primary, Secondary and Streptavidin Conjugates

HiLyte Fluor™ 405 conjugates, whether conjugated to primary tag, secondary antibodies or streptavidin, are prepared in an optimized fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised biological function. Fluorescence signal can be observed at Ex/Em = 404/428 nm.

Table 2. A list of HiLyte Fluor™ 405 labeled conjugates.

AnaTag™ HiLyte Fluor™ 405 Labeling Kits – Protein (IgG) labeling in 4 easy steps.

Table 3. AnaTag™ HiLyte Fluor™ 405 Protein Labeling Kits are available in 2 labeling sizes.

Alpha tubulin in 3T3 cells probed with mouse anti -alpha tubulin antibody, visualized with HiLyte Fluor™ 405 conjugated goat anti-mouse IgG (cat# 28176-H405).

AnaSpec is pleased to highlight the newly developed HiLyte Fluor™ 405 (in a variety of reactive forms), HiLyte Fluor™ 405 labeled conjugates and AnaTag™ HiLyte Fluor™ 405 protein labeling kits. These products are of the highest quality, and are cost-effective.