HiLyte™ Fluor 405 - NEW


AnaSpec is pleased to introduce the newest member of the HiLyte™ Fluor family
- HiLyte™ Fluor 405 (Ex/Em=404/428 nm), a bright blue fluorescent probe.

HiLyte™ Fluor dyes are a series of superior fluorescent labeling dyes that span the full visible spectrum. They are optimized for maximum brightness when conjugated with macromolecules.

Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, HiLyte™ Fluor 405 allows for easy conjugation to biopolymers. HiLyte™ Fluor 405 has spectral characteristics similar to those of Alexa Fluor™ 405 (Invitrogen) and DyLight Fluor™ 405 (Pierce) dyes with excitation and emission wavelength at 404/428 nm in PBS buffer (pH 7.4) (see spectra below). Conjugates of HiLyte™ Fluor 405 are well excited by the 407 nm spectral line of the Krypton laser and the 408 nm line of the violet laser diode.

Table 1. HiLyte™ Fluor 405 is available in different reactive forms.

Product

Size

Catalog #

HiLyte™ Fluor 405 acid NEW

10 mg

89316

HiLyte™ Fluor 405 acid, SE NEW

1 mg

89317-1

HiLyte™ Fluor 405 acid, SE NEW

5 mg

89317-5

HiLyte™ Fluor 405 amine TFA salt NEW

1 mg

89318

HiLyte™ Fluor 405 C2 maleimide NEW

1 mg

89320

HiLyte™ Fluor 405 hydrazide NEW

1 mg

89319

HiLyte™ Fluor 405 Labeled Primary, Secondary and Streptavidin Conjugates

HiLyte™ Fluor 405 conjugates, whether conjugated to primary tag, secondary antibodies or streptavidin, are prepared in an optimized fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised biological function. Fluorescence signal can be observed at Ex/Em = 404/428 nm.

Table 2. A list of HiLyte™ Fluor 405 labeled conjugates.

Product

Size

Catalog #

Goat anti - mouse IgG (H+L), highly cross - adsorbed, HiLyte™ Fluor 405 - labeled NEW

500 µg

61057-H405

Goat anti - mouse IgG (H+L), HiLyte™ Fluor 405 labeled NEW

500 µg

28175-H405

Goat anti - rabbit IgG (H+L), highly adsorbed, HiLyte™ Fluor 405 labeled NEW

500 µg

61056-H405

Goat anti - rabbit IgG (H+L), HiLyte™ Fluor 405 labeled NEW

500 µg

28176-H405

Rabbit anti - goat IgG (H+L), HiLyte™ Fluor 405 labeled NEW

500 µg

28168-H405

Rabbit anti - mouse IgG (H+L), HiLyte™ Fluor 405 labeled NEW

500 µg

28164-H405

Rabbit–anti chicken IgY (H+L), HiLyte™ Fluor 405 labeled NEW

500 µg

29709-H405


Anti - Actin (alpha Smooth muscle specific), HiLyte™ Fluor 405 - labeled NEW
Host: rabbit polyclonal; Species Reactivity: human, mouse; Application: WB, IHC

50 µg

29553-H405

Anti - GST Tag, HiLyte™ Fluor 405 labeled NEW
Host: mouse Monoclonal; Species Reactivity: all species; Application: WB, IHC, IP

100 µg

29531-H405

Monoclonal Anti - His Tag IgG, HiLyte™ Fluor 405 - labeled NEW
Host: Mouse Monoclonal; Species Reactivity: all species; Application: WB, IHC

100 µg

61250-H405

Polyclonal Anti - His Tag IgG, HiLyte™ Fluor 405 - labeled NEW
Host: rabbit; Species Reactivity: all species; Application: WB, IHC

100 µg

29673-H405


Streptavidin, HiLyte™ Fluor 405 labeled NEW

1 mg

60672-H405

AnaTag™ HiLyte™ Fluor 405 Labeling Kits – Protein (IgG) labeling in 4 easy steps.

Table 3. AnaTag™ HiLyte™ Fluor 405 Protein Labeling Kits are available in 2 labeling sizes.

Product

Catalog #

AnaTag™ HiLyte™ Fluor 405 Microscale Protein Labeling Kit *Ultra Convenient*

72142

AnaTag™ HiLyte™ Fluor 405 Protein Labeling Kit *Ultra Convenient*

72143



Alpha tubulin in 3T3 cells probed with mouse anti -alpha tubulin antibody, visualized with HiLyte™ Fluor 405 conjugated goat anti-mouse IgG (cat# 28176-H405).

AnaSpec is pleased to highlight the newly developed HiLyte™ Fluor 405 (in a variety of reactive forms), HiLyte™ Fluor 405 labeled conjugates and AnaTag™ HiLyte™ Fluor 405 protein labeling kits. These products are of the highest quality, and are cost-effective.