AnaSpec is
pleased to announce the release of a zebrafish specific p53 antibody, the Z-Fish™
Anti-p53. This antibody was tested in
fluorescent western blot analysis using HiLyte Fluor™
750 shows an immunoreactive band 53 kD, this band was also abrogated upon addition
of the immunizing peptide (Figure 1).
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Figure 1. Fluorescent
western blot analysis of zebrafish lysate (5 dpf) with Z-Fish™ Anti-p53
(CT), cat# 55342. An imunoreactive band at ~53 kDa was detected (A); this
band was blocked by the immunizing peptide (B). HiLyte Fluor™
750 –conjugated secondary antibody was used and the signals were detected
with an infrared-imaging system. |
Anti-p53, Human or Mouse Specific Antibodies
One
of the most important mammalian cell cycle checkpoint proteins is the tumor
suppressor protein, p53. In normal, undamaged cells, p53 is rapidly degraded.
However, when cells are treated with DNA damage-inducing agents, there is a
transient accumulation of p53 protein and it is activated as a transcription
factor. In several types of human cancers, p53 is mutated.1,2 Human
p53 protein has been shown to be phosphorylated at several N-terminal and C-terminal
sites that affect site-specific DNA binding and interaction with other cellular
and viral proteins in vitro.3-8 Phosphorylation at serines 6, 9,
15, 20, 33, 37 occurs after cells are exposed either to ionizing radiation or
to UV light.9,10 Serines 6 and 15 were demonstrated to be among the
strongest and earliest phosphorylated sites in response to DNA damage-induced
posttranslational modifications.11,12 AnaSpec’s collection of p53
antibody products includes both phosphospecific and non-phosphospecific solutions.
| Product |
Size |
Catalog
# |
| Anti-p53 (CT)
Host:rabbit polyclonal; Species Reactivity: human, mouse,
rat; Application: WB |
50 µg |
54421 |
| Anti-p53 (pSer6)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
25 µg
50 µg |
28006-025
28006 |
| Anti-p53 (paired6)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
50 µg |
28007 |
| Anti-p53 (pSer9)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
25 µg
50 µg |
28008-025
28008 |
| Anti-p53 (paired9)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
50 µg |
28009 |
| Anti-p53 (pSer15)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
25 µg
50 µg |
28010-025
28010 |
| Anti-p53 (paired15)
Host: rabbit polyclonal; Species Reactivity: human; Application:
WB |
50 µg |
28011 |
| Anti-p53 (pSer20)
Host:rabbit polyclonal; Species Reactivity: human; Application:
WB |
25 µg
50 µg |
54427-025
54427 |
| Anti-p53 (paired20)
Host:rabbit polyclonal; Species Reactivity: human, monkey;
Application: WB |
50 µg |
55014 |
| Anti-p53 (pSer37)
Host:rabbit polyclonal; Species Reactivity: human; Application:
Dot blot |
25 µg
50 µg |
54428-025
54428 |
| Anti-p53 (paired37)
Host:rabbit polyclonal; Species Reactivity: human, monkey;
Application: WB |
50 µg |
55015 |
| Anti-p53 (pSer46)
Host:rabbit polyclonal; Species Reactivity: human; Application:
Dot blot |
25 µg
50 µg |
54430-025
54430 |
| Anti-p53 (paired46)
Host:rabbit polyclonal; Species Reactivity: human, monkey;
Application: WB |
50 µg |
55016 |
| Anti-p53 (pSer392)
Host:rabbit polyclonal; Species Reactivity: human; Application:Dot
blot |
25 µg
50 µg |
54429-025
54429 |
| Anti-p53 (paired392)
Host:rabbit polyclonal; Species Reactivity: human, monkey;
Application: WB |
50 µg |
55017 |
| Anti-p53 (pSer18/23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application:
Dot blot |
50 µg |
55038 |
| Anti-p53 (paired18/23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey;
Application: WB |
50 µg |
55039 |
| Anti-p53 (pSer23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application:
Dot blot |
50 µg |
55036 |
| Anti-p53 (paired23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey;
Application: WB |
50 µg |
55037 |
| Anti-p53 (pSer18), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application:
WB |
50 µg |
55034A |
| Anti-p53 (paired18), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey;
Application: WB |
50 µg |
55035A |
References:
1. Brown, JM. et al. Cancer Res. 59, 1391(1999).
2. Albrechtsen, N. et al. Oncogene 18, 7706 (1999).
3. Wang, L. et al. J. Biol Chem. 276, 43604 (2001).
4. Xirodimas, D. et al. Oncogene 20, 4972 (2001).
5. Backlund, MG. et al. Cancer Res. 61, 6577 (2001).
6. Sakaguchi, K. et al. J. Biol. Chem. 275, 9278 (2000).
7. Banin, S. et al. Science 281, 1674 (1998).
8. Canman, CE. et al. Science 281, 1677 (1998).
9. Burns, TF. and El-Deiry, WS. J. cell Physiol. 181, 231 (1999).
10. Oren, M. et al. J. Biol. Chem. 274, 36031 (1999).
11. Lakin, ND. et al Oncogene 18, 7644 (1999).
12. Higashimoto, Y. et al. J. Biol. Chem. 275, 23199 (2000).
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