AnaSpec, the
provider of the ultra-sensitive SensoLyte® 520 Renin
Assay Kit is pleased to announce the release of a rat renin specific assay
– the SensoLyte® 520 Rat Renin Assay Kit. Using our proprietary 5-FAM/QXL™ 520
fluorescence resonance energy transfer (FRET) peptide, this assay is ideal for
high throughput screening of renin inhibitors and for continuous assay of rat
renin activity. In the intact FRET peptide, the fluorescence of 5-FAM is quenched
by QXL™ 520. However, upon cleavage into two separate fragments by rat renin,
the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission
= 490/520 nm. With a high fluorescence quantum yield and long emission wavelength,
the signal of 5-FAM can be detected with less interference from the autofluorescence
of cell components and test compounds. The assays are performed in a convenient
96-well microplate format and can be adjusted to 384-well microplate format.
The renin–angiotensin
system (RAS) plays a central role in the regulation of blood pressure and electrolyte
homoeostasis.1 At the first and rate-limiting step of the RAS cascade,
renin, a highly specific aspartyl protease, cleaves angiotensinogen, produced
in the liver, to yield angiotensin I, which is further converted into angiotensin
II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood
vessels leading to increased blood pressure. It also increases the secretion
of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst
reflex. Since an overactive renin-angiotensin system leads to hypertension,
renin is an attractive target for the treatment of this disease. 2-4
| |
SensoLyte™ 520 Rat Renin Assay Kit
(cat# 72140) |
SensoLyte™ 520 Renin Assay Kit (cat#
72040) |
| FRET pair |
5-FAM/QXL™ 520 |
5-FAM/QXL™ 520 |
| Ex/Em (nm) |
490/520 |
490/520 |
| Min. Detectable Renin |
0.9 ug/ml |
0.8 ng/ml |
| Interference from Autofluorescence |
Minimal |
Minimal |
| Substrate Species Reactivity |
Rat |
Human |
| Figure 1. After incubation with a renin inhibitor, Ac-HPFV-
(Sta)-LF-NH2 (cat# 60463-1), rat renin activity was measured using the SensoLyte®
520 Rat Renin Assay Kit (cat# 72140). |
Related Products:
Rat
Renin, Recombinant (20 ug) NEW
Human
Renin, Recombinant (5ug)
SensoLyte®
520 Renin Assay Kit
SensoLyte®
390 Renin Assay Kit
SensoLyte®
390 ACE2 Activity Assay Kit
Angiotensins
and Related GO™ Peptides
References:
1. He, FJ. and GA. MacGregor,
J. Renin Angiotensin Aldosterone Syst. 4, 11 (2003).
2. Wood, JM. et al., Hypertension,
7, 797 (1985).
3. Shibasaki, M. et al., Am.
J. Hypertens. 4, 932 (1991).
4. Wood, JM.et
al., Biochem. Biophys. Res. Comm. 308,
698 (2003).
5. Hui, KY.et
al., J. Med. Chem. 31, 1679 (1988).
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