The hallmark of Alzheimer’s disease (AD) pathology includes βsheet aggregates of β-amyloid peptides in senile plaques and hyperphosphorylated tau protein in neurofibrillary tangles (NFT).^1-2 AnaSpec is pleased to announce the release of 4 additional new phosphospecific anti-Tau antibodies. This represents the industry’s largest collection of anti-Tau antibodies - 21 phosphospecific and 13 non-phosphospecific antibodies.
These antibodies have been validated in dot blots and IHC (Figure 1). AnaSpec gratefully acknowledges Dr. Claudia Schwab for performing the Anti-Tau antibodies IHCs (in Dr. Patrick McGeer’s lab, University of British Columbia, Vancouver, BC, Canada).

Tau is a collection of microtubule-associated proteins that is involved in microtubule assembly and stabilization.³ In adult human brain, 6 isoforms, ranging between 352 and 441 amino acids in length, are produced as a result of alternative RNA splicing.^4-5 The expression of Tau isoforms is developmentally regulated, as only the smallest Tau polypeptide is expressed in the fetal brain. Hyperphosphorylated Tau is the major component of the paired helical filament of Alzheimer’s disease. Anti-phospho-Tau antibodies are used to identify specific amino acids that are phosphorylated in Tau from normal brains and Alzheimer’s disease brains. The Tau proteins, especially in developing brains and in Alzheimer brains, are phosphorylated in vivo at many different sites such as Thr181, Ser198, Ser199, Ser202, Thr205, Thr212, Ser214, Thr217, Thr231, Ser235, Ser262, Ser356, Ser396, Ser400, Ser404 and Ser413.^6-7

Rabbit anti-Phospho-Tau antibodies were raised against synthetic phosphopeptides. These antibodies were evaluated for specificity with ELISA and/or Western blot. By Western blot, an immunoreactive band around 52 kDa was observed in the mouse whole brain lysate. Species reactivity includes human, mouse and rat. Some antibodies have also shown reactivity with bovine, chicken and zebrafish.

Complementing one of the world’s most comprehensive collections of βamyloid peptides, the availability of these anti-Tau antibodies demonstrates AnaSpec’s commitment to delivering integrated solutions for Alzheimer research.

Figure 3. Dot blot of Tau antibodies.


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Table 1. NEW Tau Antibodies

Table 2. A full listing of AnaSpec’s Tau Antibodies

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References

1. Khachaturian, ZS. Arch. Neurol. 42, 1097 (1985).
2. Mirra, SS. et al. Neurol. 41, 479 (1991).
3. Cleveland, DW. et al. J. Mol. Biol. 116, 207 (1977).
4. Goedert, M. et al. Neuron 3, 519 (1989).
5. Geodert, M. et al. EMBO J. 8, 393 (1989).
6. Billingsley, M. et al. Biochem J. 323, 577 (1997).
7. Hanger, DP. et al. J. Neurochem. 71, 2465. (1998).