SensoLyte® 520 β- Secretase Assay Kit
As one of the world's largest providers of β-amyloid peptides and Alzheimer's related research products, AnaSpec is committed to providing tools to facilitate the advancement of Alzheimer research. One such tool is the SensoLyte® 520 β-Secretase Assay Kit.
SensoLyte® 520 β-Secretase Assay Kit
  • Fluorimetric FRET-based assay
  • Sensitive - peptide sequence based on Swedish mutation1
  • Long wavelength (Ex/Em=490/520nm), minimal autofluorescence
  • Homogeneous, mix and read assay
  • Easy to use
  • Adaptable for use in 384 or 1536 well format with minor modifications

β-Secretase is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing β-amyloid peptides through proteolytic cleavage of the β-amyloid precursor protein (APP). Accumulation of neurotoxic β-Amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer’s disease (AD), an age-related cognitive disorder.2-5

beta-Secretase FRET
Figure 1. In the FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520 until this peptide is cleaved into two separate fragments by β-secretase at the Leu-Asp bond. Upon cleavage, the fluorescence of HiLyte™ Fluor 488 is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm

Product

Catalog #

SensoLyte® 520 β-Secretase Assay Kit *Fluorimetric*

71144

 

BACE1 Sensitivity graph   Figure 2. Sensitivity of the SensoLyte® 520 β-Secretase Assay Kit (Cat# 71144). BACE, at different dilutions, was incubated with the FRET substrate at 37C and fluorescence measured after 40 min (FlexStation 382II, Molecular Devices). Sensitivity of the assay was 0.03 mU/µl.


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References:

1.    Mullan, M. et al. Nat Genet 1, 345 (1992).
2.    Vassar, R. et al. Science 286, 735 (1999).
3.    Glenner, GG. et al. Biochem Biophys Res Commun 120, 885 (1984).
4.    Selkoe, DJ. Nature 399, A23 (1999).
5.    Sinha, S. et al. Proc Natl Acad Sci USA 96, 11049 (1999).