As the world’s largest provider
of β-amyloid peptides and Alzheimer’s related research products, AnaSpec
has always been on the forefront in the development of novel solutions for this
important research field. We are pleased to announce our latest offering – the
human recombinant β-secretase enzyme.
β-secretase
(BACE1) is a key protease involved in the production of β-amyloid peptides
found in extracellular amyloid plaques of patients with Alzheimer’s disease
(AD).1 This enzyme has been implicated as an important target for
anti-amyloid therapy of AD.2
The recombinant
human BACE1 contains the catalytic domain and a C-terminal His tag. The protein
was expressed as inclusion bodies in E. coli. It was refolded and purified
using ion exchange and IMAC chromatography.3 Enzyme activity can
been measured in fluorescence resonance energy transfer (FRET) substrate based
assays (e.g. SensoLyte® 520 β-Secretase Assay Kit, cat# 71144). 100 ng
of β-secretase is sufficient for this FRET-based assay.
Figure 1. Schematic diagram of proteolytic cleavage of a FRET
peptide substrate by β-Secretase.
AnaSpec’s SensoLyte™
520 β-Secretase Assay Kit (cat# 71144) is the industry’s only long
wavelength assay kit in the green range. This kit is a fluorimetric assay kit
and is sensitive, robust and easy-to-use. It employs a FRET peptide (mentioned
above), facilitating high throughput screening of β-secretase targeting
drug candidates. The sequence of this FRET peptide is derived from the β-secretase
cleavage site of beta-amyloid precursor protein (APP) with Swedish mutation.
This mutation enhances the susceptibility of APP to β-secretase and
results in an early onset of AD.
Click
here for more information.
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of β-Amyloid GO™ Peptides
References:
- Willem, M. et al.
Semin
Cell Dev Biol. 20,175 (2009).
- Ghosh, K. et al.
Neurotherapeutics 5, 399 (2008).
- Tomasselli, G. et al. Protein
& Peptide Letters. 15,131 (2008).
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