HiLyte Fluor™ 750 – NIR Dye for Imaging


Spectrally similar to Cy7™ dye, HiLyte™ Fluor 750 dye is the longest-wavelength HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores, including HiLyte™ Fluor 647, HiLyte™ Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis. Extinction coefficient is 275,000 M-1cm-1, with a quantum yield of 0.12. With a peak excitation at ~753 nm, conjugates of HiLyte™ Fluor 750 dyes can be excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. Its near infrared spectra render the HiLyte™ Fluor 750 dye the ideal candidate for in vitro and in vivo tissue imaging.

Figure 1. Absorption and emission spectra of HiLyte™ Fluor 750.
Figure 2. In-vivo image of a rat injected with 20 nmol of c[RGDyK(HiLyte™ Fluor 750)] peptide-dye conjugate 3h post injection (image courtesy of J. Rey, Univ. of South Florida).
Figure 3. Western blot analysis of zebrafish lysate (5 dpf) with Z-Fish™ anti-p53 (CT), cat# 55342. An imunoreactive band at ~53 kDa was detected (A); this band was blocked by the immunizing peptide (B). HiLyte™ Fluor 750 –conjugated secondary antibody, cat# 28176-H750 was used and signals detected with an infrared-imaging system (Odyssey, Li-Cor, Inc).


Table 1. HiLyte™ Fluor 750 is available in various reactive forms.

Product

Size

Catalog #

HiLyte™ Fluor 750 acid

5 mg

81265

HiLyte™ Fluor 750 acid, SE

1 mg

81266

HiLyte™ Fluor 750 amine

1 mg

81267

HiLyte™ Fluor 750 hydrazide

1 mg

81268

HiLyte™ Fluor 750 C2 maleimide

1 mg

81269

HiLyte™ Fluor 750 Bis-NHS ester, isomer II TEA salt

1 mg

81270


Table 2. HiLyte™ Fluor 750 labeled secondary antibodies.

Product

Size

Catalog #

Goat anti-mouse IgG (H+L), HiLyte™ Fluor 750-labeled

500 ug

28175-H750

Goat anti-mouse IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 750-labeled

500 ug

61057-H750

Goat anti-rabbit IgG (H+L), HiLyte™ Fluor 750-labeled

500 ug

28176-H750

Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor 750-labeled

500 ug

61056-H750

Rabbit anti-chicken IgY (H+L), HiLyte™ Fluor 750-labeled

500 ug

29709-H750

Rabbit anti-mouse IgG (H+L), HiLyte™ Fluor 750-labeled

500 ug

28164-H750

Rabbit anti-goat IgG (H+L), HiLyte™ Fluor 750-labeled

500 ug

28168-H750

Streptavidin, HiLyte™ Fluor 750 conjugated

1 mg

60659-H750


Table 2. AnaTag™ HiLyte™ Fluor 750 protein (IgG) labeling kits.

Product

Size

Catalog #

AnaTag™ HiLyte™ Fluor 750 Microscale Protein Labeling Kit *Ultra Convenient*

1 kit

72044

AnaTag™ HiLyte™ Fluor 750 Protein Labeling Kit *Ultra Convenient*

1 kit

72043