The vertebrate zebrafish (Danio rerio) is increasingly used as an animal model in the study of vertebrate development and for understanding human disease processes.^1-2 With the increased use of the zebrafish model there has been a corresponding demand for zebrafish specific reagents. AnaSpec is pleased to be the industry’s first provider of Z-Fish™ antibodies, specifically for zebrafish target proteins.

Our expanding list of Z-Fish™ antibodies includes targets used in apoptosis and autophagy, cell cycle control, cytoskeleton, glucose and energy metabolism, signal transduction, and immunity research. Antibodies were verified in ELISA and in western blots, using zebrafish lysates. All Z-Fish™ antibodies are predicted to work in IHC, with over half of them showing good results in IHC. Supplied in 150 ul size, this amount is enough for 15 mini gels.

Examples of antibodies for zebrafish signal transduction are the NFkB and related antibodies. In humans, nuclear factor kappa beta (NFkB) complexes are held in cytoplasm and bound to members of the NFkB inhibitor family (IkB).³ Studies on other animal models and the inactivation of the NFkB pathway show severe defects in embryonic development and prenatal death.³ One study demonstrated high conservation and compatibility of NFkB and IkB proteins across vertebrate species in utilizing murine IkB to interfere with the NFkB pathway in zebrafish embryonic development.³ NFkB and Snail1a coordinate the cell cycle through gastrulation.⁴ The IkB kinase (IKK) activity, which processes IkB proteins, negatively regulates NFkB and can function as a repressor.⁵

Figure 1. Western blot (WB) of NFKB-2 using Z-Fish™ embryonic lysate (24 hpf), cat# 55347, probed with Z-Fish™ anti-NFKB-2 (IN), cat# 55484. An immunoreactive band at ~48 kDa was detected; band was blocked by the immunizing peptide (B). Right panel is a fluorescent WB, secondary antibody used was HiLyte Fluor™ 750 –goat anti-rabbit, cat# 28176-H750.

Figure 2. Western blot of Rel protein using Z-Fish™ larva lysate (5 dpf), cat# 55350, probed with Z-Fish™ anti-Rel (IN), cat# 55483. An immunoreactive band at ~65 kDa was detected (A); band was blocked by the immunizing peptide (B). Right panel is a fluorescent WB, secondary antibody used was HiLyte Fluor™ 750 –goat anti-rabbit, cat# 28176-H750.

Figure 3. Z-Fish™ embryo (72 hpf), cat# 55349 stained with Z-Fish™ anti-Rela (NT), cat# 55482. Staining was blocked by the immunizing peptide (B).

Figure 4. Western blot of Rela protein using Z-Fish™ larva lysate (5 dpf), cat# 55350, probed with Z-Fish™ anti-Rela (NT), cat# 55482. An immunoreactive band at ~51 kDa was detected (B); the band was blocked by the immunizing peptide (D).

Figure 5. Western blot of Nfkbi-alpha B using Z-Fish™ larva lysate (5 dpf), cat# 55350 with Z-Fish™ anti-Nfkbi-alpha B (NT), cat# 55481. An Immunoreactive band at ~35 kDa was detected; band was blocked by the immunizing peptide (B). Right panel is a fluorescent WB, secondary antibody used was HiLyte Fluor™ 750 –goat anti-rabbit, cat# 28176-H750.

Related Products

Full listing of Z-Fish™ Signal Transduction Antibodies

Full listing of Z-Fish™ Antibodies

Full listing of Z-Fish™ Lysates

Reference

1. Grunwald, DJ. & JS Eisen, Nat. Rev. Genet. 3, 717 (2002).
2. Correa, RG et al. Mol. Cell. Biol. 24, 5257 (2004).
3. Hertlein, E et al. Mol. Cell. Bio. 25(12): 4956 (2005).
4. Liu, X et al. J. Cell Biol. 184(6): 805 (2009).
5. Correa, RG et al. Curr. Biol. 15(14): 1291 (2005).