AnaSpec, Eurogentec’s newest North American division, is pleased
to offer Eurogentec’s popular monoclonal antibody, anti-5-methylcytidine (Clone
33D3) for use in epigenetics research. Purified from ascites, this IgG1 / λ isotype antibody is supplied as a 1 mg/ml solution (in PBS) and
is available in three sizes (0.1 mg, 0.5 mg and 1 mg).
5-Methylcytidine is a modified base found in the DNA of plants and
vertebrate. DNA methylation is a post-replication process involved in the establishment
of genomic imprinting, in the control of gene expression and of differentiation.
Alterations of DNA methylation pattern are associated with carcinogenesis. A
global DNA hypomethylation is often detected in tumor tissues, associated with
local hypermethylation sites. Since this antibody discriminates between the
modified base, 5-MeCyd and
its normal counterpart, cytidine; it
is a useful tool for gene promoter methylation analysis.
The most published anti 5-Methylcytidine
antibody available on the market,
anti-5-Methylcyidine from Clone 33D3 can be used in applications such as Methylated DNA Immunoprecipitation (MeDIP), MeDIP-on-chip
assay, immunofluorescence, flow cytometry and immunohistochemistry. Histopathological analysis of tissue samples can be
performed without having to destroy cells through DNA extraction procedures.
This antibody has been used to detect alterations in the urinary
excretion of nucleosides from cancer patients, to visualize the distribution
of methyl-rich regions along human chromosomes, to quantify in situ differences
between normal and malignant cells from peripheral blood as well as on tissue
sections.
| Product |
Size |
Catalog # |
| Monoclonal Antibody against 5-Methylcytidine
NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC,
IHC, and Cytogenetics |
0.1 mg |
BI-MECY-0100 |
| Monoclonal
Antibody against 5-Methylcytidine NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC,
IHC, and Cytogenetics |
0.5 mg |
BI-MECY-0500 |
| Monoclonal
Antibody against 5-Methylcytidine NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC,
IHC, and Cytogenetics |
1 mg |
BI-MECY-1000 |
The following papers are examples
of recent publications citing the use of this antibody. To view a citation archive,
please click here.
Jin, SG. et al.
(2010). Examination of the specificity of DNA methylation profiling techniques
towards 5-methylcytosine and 5-hydroxymethylcytosine. Nucleic Acid Res.
doi:10.1093/nar/gkq22.
Sanchez-Abarca,
LI. et al. (2010). Immunomodulatory effect of 5-azacytidine (5-azaC): potential
role in the transplantation setting. Blood 115: 107, doi: 10.1182/blood-2009-03-210393.
Wolk, M. & JE. Martin
(2009). Titrimetric immunohistochemical evaluation of DNA hypomethylation
in uterine tumours. J. Clin. Pathol. 62:1039, doi:10.1136/jcp.2009.066613.
Peters, DD. et al.
(2009). Mammalian Genome 20: 664, doi: 10.1007/s00335-009-9227-0.
Mutskov, V. & G.
Felsenfeld (2009). The human insulin gene is part of a large open
chromatin domain specific for human islets. Proc.
Natl. Acad. Sci. 106: 17419, doi: 10.1073/pnas.0909288106.
Other epigenetics products
include a wide selection of histone peptides, especially histone H3 peptides,
SensoLyte® HDAC, LSD1 and SIRT1 assay kits.
Related Products:
Histone GO™
Peptides – full listing
Histone
H3 GO™ Peptides
SensoLyte®
HDAC Assay Kits, Fluorimetric
SensoLyte® LSD1 Assay
Kit, Fluorimetric
SensoLyte® SIRT1 Assay
Kits, Fluorimetric
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