Fluorescent Ca2+ Indicators Premium Quality


Calcium plays a vital role in the regulation of cardiac contractility, growth and gene expression; consequently, calcium measurement is critical in numerous biological investigations. Fluorescent probes that show spectral responses upon Ca2+ binding have enabled researchers to investigate changes in intracellular free Ca2+ concentrations using techniques such as fluorescence microscopy, flow cytometry, fluorescence spectroscopy. Most of these fluorescent indicators are derivatives of BAPTA, chelators that incorporate a photo-induced-electron transfer (PET) system responsive to calcium. Instruments, such as FLIPR and FlexStationTM have enabled high throughput measurement of calcium for GPCR and ion channel research.

AnaSpec is pleased to highlight a wide selection of fluorescent Ca2+ indicators. These calcium indicators are of the highest quality and are cost-effective. They include UV-excitable and visible light-excitable calcium indicators.

Among the UV-excitable calcium indicators, Fura-2 and Indo-1 are the most commonly used. Fura-2 is excitation-ratioable while indo-1 is emission-ratioable. While Fluo-3 and Rhod-2 are the most commonly used among the visible light-excitable calcium indicators. Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based HTS assays for functional GPCR assays. The long-wavelength Ca2+ indicators, Rhod-2 and X-rhod-1, are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high autofluorescence.

To inquire about licensing opportunities for AnaSpec dyes, please email service@anaspec.com.

UV-Excitable Calcium Indicators

Product Size Catalog #

Fura 2, AM

1 mg

84015

Fura - 2, AM *UltraPure Grade*

1 mg

84016

Fura - 2, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84019

Fura - 2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mL

84018

Fura - 2, pentapotassium salt

1 mg

84011

Fura - 2, pentasodium salt

1 mg

84012

Fura - 2; AM *UltraPure Grade* *Small Package*

50 g x 20

84017

Indo - 1, AM

1 mg

84006

Indo - 1, AM *UltraPure Grade*

1 mg

84007

Indo - 1, AM *UltraPure Grade* *Bulk packaging*

50 mg

84010

Indo - 1, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mg

84009

Indo - 1, pentapotassium salt

1 mg

84000

Indo - 1, pentasodium salt

1 mg

84001

Indo - 1; AM *UltraPure Grade* *Small Package*

50 g x 20

84008

Indo - 5F, AM

1 mg

84051

Indo - 5F, pentapotassium salt

1 mg

84050


Visible Light-Excitable Calcium Indicators

Product

Size

Catalog #

Fluo - 3, AM

1 mg

84024

Fluo - 3, AM *UltraPure Grade*

1 mg

84025

Fluo - 3, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84028

Fluo - 3, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mL

84027

Fluo - 3, pentaammonium salt

1 mg

84020

Fluo - 3, pentapotassium salt

1 mg

84021

Fluo - 3, pentasodium salt

1 mg

84022

Fluo - 3; AM *UltraPure Grade* *Small Package*

50 g x 20

84026

Fluo 5F, pentapotassium salt

1 mg

84055

Fluo 5F, AM

1 mg

84056

Fluo 5N, pentapotassium salt

1 mg

84057

Fluo 5N, AM

1 mg

84058

Rhod - 2, AM

1 mg

84034

Rhod - 2, AM *UltraPure Grade*

1 mg

84035

Rhod - 2, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84038

Rhod - 2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mg

84037

Rhod - 2, tripotassium salt

1 mg

84030

Rhod - 2, trisodium salt

1 mg

84031

Rhod - 2; AM *UltraPure Grade* *Small Package*

50 g x 20

84036

Rhod - 5F, AM

1 mg

84060

Rhod - 5F, tripotassium salt

1 mg

84059

Rhod - 5N, AM

1 mg

84062

Rhod - 5N, tripotassium salt

1 mg

84061

X - Rhod - 1, tripotassium salt

1 mg

84049

X - Rhod - 1, AM

1 mg

84048