Fluorescent Ca2+ Indicators – Premium Quality


Calcium plays a vital role in the regulation of cardiac contractility, growth and gene expression; consequently, calcium measurement is critical in numerous biological investigations. Fluorescent probes that show spectral responses upon Ca2+ binding have enabled researchers to investigate changes in intracellular free Ca2+ concentrations using techniques such as fluorescence microscopy, flow cytometry, fluorescence spectroscopy. Most of these fluorescent indicators are derivatives of BAPTA, chelators that incorporate a photo-induced-electron transfer (PET) system responsive to calcium. Instruments, such as FLIPR® and FlexStationTM have enabled high throughput measurement of calcium for GPCR and ion channel research.

AnaSpec is pleased to highlight a wide selection of fluorescent Ca2+ indicators. These calcium indicators are of the highest quality and are cost-effective. They include UV-excitable and visible light-excitable calcium indicators.

Among the UV-excitable calcium indicators, Fura-2 and Indo-1 are the most commonly used. Fura-2 is excitation-ratioable while indo-1 is emission-ratioable. While Fluo-3 and Rhod-2 are the most commonly used among the visible light-excitable calcium indicators. Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based HTS assays for functional GPCR assays. The long-wavelength Ca2+ indicators, Rhod-2 and X-rhod-1, are valuable alternatives to Fluo-3 for experiments in cells and tissues that have high autofluorescence.

To inquire about licensing opportunities for AnaSpec dyes, please email service@anaspec.com.

UV-Excitable Calcium Indicators

Product Size Catalog #

Fura – 2, AM

1 mg

84015

Fura - 2, AM *UltraPure Grade*

1 mg

84016

Fura - 2, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84019

Fura - 2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mL

84018

Fura - 2, pentapotassium salt

1 mg

84011

Fura - 2, pentasodium salt

1 mg

84012

Fura - 2; AM *UltraPure Grade* *Small Package*

50 µg x 20

84017

Indo - 1, AM

1 mg

84006

Indo - 1, AM *UltraPure Grade*

1 mg

84007

Indo - 1, AM *UltraPure Grade* *Bulk packaging*

50 mg

84010

Indo - 1, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mg

84009

Indo - 1, pentapotassium salt

1 mg

84000

Indo - 1, pentasodium salt

1 mg

84001

Indo - 1; AM *UltraPure Grade* *Small Package*

50 µg x 20

84008

Indo - 5F, AM

1 mg

84051

Indo - 5F, pentapotassium salt

1 mg

84050


Visible Light-Excitable Calcium Indicators

Product

Size

Catalog #

Fluo - 3, AM

1 mg

84024

Fluo - 3, AM *UltraPure Grade*

1 mg

84025

Fluo - 3, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84028

Fluo - 3, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mL

84027

Fluo - 3, pentaammonium salt

1 mg

84020

Fluo - 3, pentapotassium salt

1 mg

84021

Fluo - 3, pentasodium salt

1 mg

84022

Fluo - 3; AM *UltraPure Grade* *Small Package*

50 µg x 20

84026

Fluo – 5F, pentapotassium salt

1 mg

84055

Fluo – 5F, AM

1 mg

84056

Fluo – 5N, pentapotassium salt

1 mg

84057

Fluo – 5N, AM

1 mg

84058

Rhod - 2, AM

1 mg

84034

Rhod - 2, AM *UltraPure Grade*

1 mg

84035

Rhod - 2, AM *UltraPure Grade* *Bulk Packaging*

50 mg

84038

Rhod - 2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO

1 mg

84037

Rhod - 2, tripotassium salt

1 mg

84030

Rhod - 2, trisodium salt

1 mg

84031

Rhod - 2; AM *UltraPure Grade* *Small Package*

50 µg x 20

84036

Rhod - 5F, AM

1 mg

84060

Rhod - 5F, tripotassium salt

1 mg

84059

Rhod - 5N, AM

1 mg

84062

Rhod - 5N, tripotassium salt

1 mg

84061

X - Rhod - 1, tripotassium salt

1 mg

84049

X - Rhod - 1, AM

1 mg

84048