MESA BLUE qPCR Mixes for SYBR Assay


Eurogentec’s MESA BLUE qPCR MasterMix Plus for SYBR® Assay is a unique combination of our high-performance Hotstart MeteorTaq and an inert BLUE dye. The BLUE dye, which does not affect the qPCR reaction, considerably enhances the contrast between the reagent and plasticware, making verification of MasterMix dispensing quick, easy and foolproof, especially when using white plates.

MESA BLUE qPCR MasterMixes, tailored for every thermocycler requirement, are ready-to-use, easy-to-see formulated mixes. These kits provide consistent and sensitive results across all different qPCR platforms and prevent aberrant results before they occur.

· For SYBR assay with both FAST and REGULAR protocols
· Visualize dispensing errors at a glance
· Optimised for maximal sensitivity
· Maximum consistency and specificity
· Easy-to-see & Easy-to-use

Ct MESA BLUE

12.69

15.74

19.5

22.96

26.88

30.5

Ct Competitor A

13.56

17.05

20.55

24.27

27.64

31.35

Figure 1. Comparison of the MESA BLUE MasterMix Plus for SYBR Assay and competitor A shows increased sensitivity, steeper amplification curves of the MESA BLUE MasterMix Plus (No ROX). Experiments were performed using the same cDNA and18S primers on a MX3000P®.

Table 1. A MESA BLUE mastermix for every thermocycler requirement.

Product

Size

Catalog #

MESA BLUE qPCR MasterMix Plus for SYBR® Assay

600 RXNs (7.5 ml)

RT-SY2X-03+WOUB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay, Low ROX

600 RXNs (7.5 ml)

RT-SY2X-03_WOULRB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay, No ROX

600 RXNs (7.5 ml)

RT-SY2X-03+NRWOUB

MESA BLUE qPCR MasterMix Plus for SYBR® Assay, w/fluorescein

600 RXNs (7.5 ml)

RT-SY2X-03+WOUFLB

* Use ROX mixes on ABI (except 7500) and Eppendorf thermocyclers. Use Low ROX mixes on ABI 7500 and Stratagene (when ROX normalization is required) machines. Use No ROX mixes on all other machines.

View the Blue MasterMixes for SYBR® and Probe Assays and Fast MasterMixes for SYBR® and Probe Assays flyers.

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