SensoLyte® HAT (p300) & HAT (pCAF) Assay Kits


AnaSpec is pleased to announce the simultaneous release of two HAT (histone acetyltransferase) fluorimetric kits to complement our growing list of epigenetics research products.

The SensoLyte® HAT (p300) Assay Kit provides a convenient assay for the screening of enzyme inhibitors and for continuous measurement of p300 activity. For added convenience, this kit includes two peptide substrates: histone H3 (1-21) peptide and non-histone p53 peptide (368-386). After incubation with acetyl CoA and the substrate, the p300 enzyme generates acetylated H3 or p53 peptide and CoASH. The thiol groups of CoASH can be detected with a fluorogenic reagent at excitation/emission=389nm/513nm.

The SensoLyte® HAT (pCAF) Assay Kit provides a convenient assay for screening of enzyme inhibitors and for continuous measurement of pCAF activity. After incubation with acetyl CoA and histone H3 (1-21) peptide, the pCAF enzyme generates acetylated H3 peptide and CoASH. The detection of the CoASH thiol group is the same as above.

Histone acetyltransferases (HATs) enzymes regulate the acetylation of histones and non-histone proteins.1, 2 The acetylation of the amino groups of lysine residues present at histone tails correlates largely with transcriptional activation, but it is also involved in DNA replication, DNA repair and protein–protein interactions.3 HATs play major roles in the control of cell fate and misregulation is implicated in the development of some human tumors.4,5 HAT p300 is a transcriptional coactivator that acetylates core histones facilitating chromatin decondensation and recruiting basic RNA polymerase machinery.6 Many non-histone proteins, such as p53, STATs, and alpha interferon receptor, also serve as substrates for p300.7The p300/CBP-associated factor (pCAF) acetylates specific lysines on the N-terminal tails of histones H3 and H4.


Figure 1. Inhibition of p300 activity (H3 peptide)was measured using SensoLyte®HAT (p300) Assay Kit.

Product

Catalog #

SensoLyte® HAT (p300) Assay Kit *Fluorimetric*

72172

SensoLyte® HAT (pCAF) Assay Kit *Fluorimetric*

72173






Recent H3 Peptide Releases
Product Size Catalog #

[Arg(Me2a)2] - Histone H3 (1 - 21) - GGK(Biotin), H3R2(Me2a), biotin - labeled NEW
A - R(Me2a) - TKQTARKSTGGKAPRKQLA - GGK(Biotin) - NH2

0.25 mg

1 mg

64630-025

64630-1

[Arg(Me2s)2] - Histone H3 (1 - 21) - GGK(Biotin); H3R2(Me2s), biotin - labeled NEW
A - R(Me2s) - TKQTARKSTGGKAPRKQLA - GGK(Biotin) - NH2

0.25 mg

1 mg

64631-025

64631-1

Arg(Me2a)17] - Histone H3 (1 - 21) - GGK(Biotin); H3R17(Me2a), biotin - labeled NEW
ARTKQTARKSTGGKAP - R(Me2a) - KQLA - GGK(Biotin) - NH2

0.25 mg

1 mg

64634-025

64634-1

[Arg(Me2s)17] - Histone H3 (1 - 21) - GGK(Biotin); H3R17(Me2s), biotin - labeled NEW
ARTKQTARKSTGGKAP - R(Me2s) - KQLA - GGK(Biotin) - NH2

0.25 mg

1 mg

64633-025

64633-1

Related Products
Large collection of Histone peptides
Acetylated H2A peptides
Acetylated H2B peptides
Acetylated H3 peptides
Acetylated H4 peptides
SensoLyte® 520 HDAC Activity Assay Kit *Fluorimetric*, Cat# 72084
SensoLyte® 440 HDAC Activity Assay Kit *Fluorimetric*, Cat# 72083
SensoLyte® LSD1 Assay Kit *Fluorimetric*, Cat# 72131
SensoLyte® 520 FRET SIRT1 Assay Kit *Fluorimetric*, Cat# 72155
SensoLyte® Green SIRT1 Assay Kit *Fluorimetric*, Cat# 72156
SensoLyte® Histone H3 (1-21) Peptides Sampler Kits, Cat# 72175, 72176

References

1. Roth, SY. et al. Annu Rev Biochem. 70, 81 (2001).
2. Glozak, MA. et al. Gene. 363, 15 (2005).
3. Turner, BM. Bioessays 22, 836 (2000).
4. Timmermann, S. et al. Cell Mol Life Sci. 58, 728 (2001).
5. Iyer, NG. et al. Oncogene. 23, 4225 (2004).
6. Chan, HM. et al. J Cell Sci. 114, 2363 (2001).
7. Grossman, SR. Eur J Biochem. 268, 2773 (2001).