AnaSpec is pleased to announce the simultaneous release of
two HAT (histone acetyltransferase) fluorimetric kits to complement our growing
list of epigenetics research products.
The SensoLyte® HAT (p300) Assay Kit
provides a convenient assay for the screening of enzyme inhibitors and for continuous
measurement of p300 activity. For added convenience, this kit includes two peptide substrates:
histone H3 (1-21) peptide and non-histone p53 peptide (368-386). After incubation
with acetyl CoA and the substrate, the p300 enzyme generates acetylated H3 or p53 peptide
and CoASH. The thiol groups of CoASH can be detected with a fluorogenic reagent
at excitation/emission=389nm/513nm.
The SensoLyte® HAT (pCAF) Assay
Kit provides a convenient assay for screening of enzyme inhibitors and for
continuous measurement of pCAF activity. After incubation with acetyl CoA and
histone H3 (1-21) peptide, the pCAF enzyme generates acetylated H3 peptide and
CoASH. The detection of the CoASH thiol group is the same as above.
Histone acetyltransferases
(HATs) enzymes regulate the acetylation
of histones and non-histone proteins.1, 2 The acetylation of the amino
groups of lysine residues present at histone tails correlates largely with transcriptional
activation, but it is also involved in DNA replication, DNA repair and protein–protein
interactions.3 HATs play major roles in the control of cell fate
and misregulation is implicated in the development of some human tumors.4,5
HAT p300 is a transcriptional coactivator that acetylates core histones
facilitating chromatin decondensation and recruiting basic RNA polymerase machinery.6
Many non-histone proteins, such as p53, STATs, and alpha interferon receptor,
also serve as substrates for p300.7The p300/CBP-associated factor (pCAF) acetylates specific
lysines on the N-terminal tails of histones H3 and H4.
Figure 1. Inhibition of p300 activity (H3 peptide)was measured using SensoLyte®HAT (p300)
Assay Kit.
Recent H3 Peptide Releases
| Product |
Size |
Catalog
# |
| [Arg(Me2a)2] - Histone H3 (1 - 21) - GGK(Biotin),
H3R2(Me2a), biotin - labeled NEW
A - R(Me2a) - TKQTARKSTGGKAPRKQLA - GGK(Biotin) - NH2 |
0.25 mg
1 mg |
64630-025
64630-1 |
| [Arg(Me2s)2] - Histone H3 (1 - 21) - GGK(Biotin);
H3R2(Me2s), biotin - labeled NEW
A - R(Me2s) - TKQTARKSTGGKAPRKQLA - GGK(Biotin) - NH2 |
0.25 mg
1 mg |
64631-025
64631-1 |
| Arg(Me2a)17] - Histone H3 (1 - 21) - GGK(Biotin);
H3R17(Me2a), biotin - labeled NEW
ARTKQTARKSTGGKAP - R(Me2a) - KQLA - GGK(Biotin) - NH2 |
0.25 mg
1 mg |
64634-025
64634-1 |
| [Arg(Me2s)17] - Histone H3 (1 - 21) - GGK(Biotin);
H3R17(Me2s), biotin - labeled NEW
ARTKQTARKSTGGKAP - R(Me2s) - KQLA - GGK(Biotin) - NH2 |
0.25 mg
1 mg |
64633-025
64633-1 |
Related Products
Large collection
of Histone peptides
Acetylated
H2A peptides
Acetylated
H2B peptides
Acetylated
H3 peptides
Acetylated
H4 peptides
SensoLyte®
520 HDAC Activity Assay Kit *Fluorimetric*, Cat# 72084
SensoLyte®
440 HDAC Activity Assay Kit *Fluorimetric*, Cat# 72083
SensoLyte®
LSD1 Assay Kit *Fluorimetric*, Cat# 72131
SensoLyte® 520 FRET SIRT1 Assay Kit *Fluorimetric*, Cat# 72155
SensoLyte®
Green SIRT1 Assay Kit *Fluorimetric*, Cat# 72156
SensoLyte® Histone H3 (1-21) Peptides Sampler Kits, Cat# 72175, 72176
References
1.
Roth, SY. et al. Annu Rev Biochem. 70, 81 (2001).
2.
Glozak, MA. et al. Gene. 363, 15 (2005).
3.
Turner, BM. Bioessays 22, 836 (2000).
4.
Timmermann, S. et al. Cell Mol Life Sci. 58, 728 (2001).
5.
Iyer, NG. et al. Oncogene. 23, 4225 (2004).
6.
Chan, HM. et al. J Cell Sci. 114, 2363 (2001).
7.
Grossman, SR. Eur J Biochem. 268, 2773
(2001).
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