Cherry™Express T7 Protein Expression Kits – direct visualization


The Cherry™Express kit allows direct visualization through the whole process of T7 protein expression, production and purification without the need for any special requirements or reagents. A simple absorbance reading at 413 nm gives specific and accurate calculation of the target protein concentration at each step.

The Cherry™Express kit provides

  • Direct Visualization
  • Plasmid and/or protein expression without the use of antibiotics
  • Increase protein solubility
  • Easy quantification
  • Easy to follow purification steps
  • Rapid screening and optimization of protein expression
  • High yield of protein production using Staby™ system*

*The Staby™ technology removes the need for traditional antibiotic selection while ensuring a 100% homogenous population of plasmid-containing cells before and after induction of protein expression.

Product

Size

Catalog #

Cherry™Express T7 expression kit (N-terminal tag), electro-competent cells

5 RXNs

10 RXNs

GE-CET7-05

GE-CET7-10

Cherry™Express T7 expression kit (C-terminal tag), electro-competent cells

5 RXNs

10 RXNs

GE-CET7-06

GE-CET7-11

Cherry™Express T7 expression kit (N-terminal tag), chemically-competent cells

5 RXNs

10 RXNs

GE-CET7-07

GE-CET7-12

Cherry™Express T7 expression kit (C-terminal tag), chemically-competent cells

5 RXNs

10 RXNs

GE-CET7-08

GE-CET7-13


Easy quantification of the protein of interest

When using the Cherry™Express, it is possible to quantify the protein concentration at any step (from protein production to the end of purification process): a simple absorbance measurement at 413 nm allows specific and accurate calculation of the target protein concentration.

The red color constitutes a visual marker throughout the protein purification steps. Concentration of the fusion protein can be determined easily by spectral measurement at 413 nm (before and after purification). After purification, the tag can be cleaved using Enterokinase (a recognition site is inserted at the C-terminal end of the tag sequence). Watch the video! http://www.youtube.com/watch?v=WSASC1HRiJg

Rapid screening and optimization of protein expression

The Cherry™Express system is convenient for rapid screening and optimization of protein solubility. The kit provides visual aid for "live" estimation of the best conditions such as time and temperature. Just follow the OD at 413 nm! There is no need to lyse cells to follow the purification process to determine if the protein is expressed or not.

Increase protein solubility

Lack of solubility is a major problem when expressing recombinant protein in E. coli. However, when using the Cherry™Express vector, the Cherry™ tag (heme binding part of cytochrome, 11 kDa) is highly soluble and thus, can increase the solubility of target proteins.

PSCherry1 and PSCherry3 vectors allow expression of protein with the Cherry™ tag at the N- or C-terminus, respectively. The Cherry™ tag inserted in the StabyExpress™ T7 vector gives multiple advantages such as T7 expression, plasmid stabilization without antibiotics and protein visualization.

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