Since our inception in 1993, AnaSpec has assisted
scientists in the design and synthesis of >50,000 peptides. As a result,
our combined knowledge and expertise provide premium solutions to our customers.
AnaSpec routinely completes difficult peptide synthesis such as synthesis of
long peptides (>90-mers), peptides containing unusual modifications, and
cyclic peptides. In addition, we have the capacity for large-scale synthesis
(>100 g).
Optimized Fmoc and Boc methodologies are employed
for peptide syntheses. Cleavage of peptides is performed using trifluoroacetic
acid (TFA), hydrogen fluoride (HF), or other appropriate methods. Most of the
peptides are purified by HPLC using reverse phase C4 and C18 columns. Alternative
purification methods (e.g., ion exchange and gel filtration chromatography)
are used when needed. AnaSpec is equipped with state-of-the-art automated peptide
synthesizers capable of performing synthesis ranging from small (0.025 mmol)
to large (50 mmol) scale. These state-of-the-art synthesizers, coupled with
our experienced peptide chemists, enable us to deliver our peptides quickly
and in high quality (purity of 90-95% is typical). Complete confidentiality of
all orders is assured.
AnaSpec’s synthesis facilities located in Fremont, California means that we
are able to provide comprehensive oversight of the entire peptide synthesis
process. This ensures that we consistently meet our customer’s product specifications.
All custom peptides are accompanied with MS and HPLC analyses. Additional analytical
data (e.g., amino acid analysis, sequencing) are available upon request. AnaSpec
is also proud to be certified to ISO 9001:2008.
Custom Peptide Modifications
| • Alkyne peptides |
| • Azide peptides |
| • BSA, KLH conjugated peptides for antibody production
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| • C-terminal modifications |
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* Aldehydes |
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* Alcohols |
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* Chloromethyl Ketones |
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* Cysteamide |
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* Ester |
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* N-Alkyl Amides |
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* Hydrazides |
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* Thio ester |
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| • Cyclic peptides |
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* N->C |
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* Disulfide (S-S bond formation) |
| • Dehydro peptides |
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* dehydroalanine; dehydroleucine, dehydroproline |
| • Dityrosine Cross linked peptides |
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| • Drug-peptide conjugates |
| • Dye-labeled peptides: |
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* C-terminal |
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* AFC, AMC, Dap(Dnp), Lys(Dye),
pNA, Rh110 |
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* N-terminal |
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* HiLyte
Flour™ dyes (405, 488, 555, 647, 680, 750) |
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* QXL™ quenchers |
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* FAM, FITC, MCA, Rox, Sulforhodamine
101, TAMRA |
| • FRET Substrates |
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* 5-FAM/QXL™ 520 |
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* HiLyte Fluor™ 488/QXL™ 520 |
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* TAMRA/QXL™ 570 |
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* EDANS/DABCYL and other FRET pairs |
| • Glycopeptides |
| • Heavy Isotope Labeled Peptides (ClearPoint peptides
labeled with D, C13, N15) |
| • Hydrocarbon Stapled Peptides |
| • Lactam peptides |
| • Lipopeptides |
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* Prenylated |
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* Farnesyl |
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* GeranylGeranyl |
| • MAPS (Multiple Antigenic Peptide) |
| • Methylated peptides |
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* Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, Arg(Me)2
assymmetrical |
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* Na-Methylated |
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* Ca-Methylated |
| • N-terminal Modifications |
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* Acetylated
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* Biotinylated
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* Bromoacetylated |
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* DOTA |
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* Formylated |
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* Myristoylated |
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* Succinylated |
| • Pegylated peptides |
| • Peptide libraries |
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* 96-well plate libraries |
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* One-Bead One-Peptide |
| • Peptidomimetics |
| • Peptide bond modifications |
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* Ester (Depsipeptides) |
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* Amine (reduced amide bond) |
| • Phosphopeptides |
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* Phosphoserine, Phosphothreonine, Phosphotyrosine |
| • Sulfated Tyrosine or Serine |
| • Thiolactone peptide |
| • TR-FRET Substrates |
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* Europium/QXL™ 610 |
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* Europium/HiLyte Fluor™ 647 |
| • Unusual Synthesis |
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For additional information about our custom peptide synthesis services, please contact service@anaspec.com
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