Calcein AM (Calcein acetoxymethyl ester), a non-fluorescent, cell permeable compound,
converts to the strongly green fluorescent calcein when hydrolyzed by intracellular
esterases in live cells (Figure 1).1-2 It is widely used for determining
cell viability since the fluorescence intensity of calcein is proportional to
the amount of live cells (Figures 2-4). Calcein is a photostable reagent and
is well retained in the cytoplasm of live cells. Calcein is not influenced by
intracellular pH. Its bright green fluorescence can be monitored at Ex/Em=494nm
/520 nm (Figure 5).
Amongst other reagents, including BCECF, AM and Carboxy-fluorescein
diacetate, Calcein AM is the most suitable indicator for staining viable cells
due to its low cytotoxicity property. Calcein AM does not affect cell proliferation
or viability.2-3 Calcein AM is used in cell invasion, adhesion, migration
and other cell-based assays.
AnaSpec is pleased to provide Calcein AM at two purity grades,
>90% or >95%, as determined by HPLC (Figure 6). AnaSpec also offers the
Calcein Cell Viability Assay Kit - a rapid and sensitive assay to detect
viable cells. This kit provides ample reagents (dye and assay buffer) for 1000
assays in a 96-well plate format.
AM (Carboxyfluorescein diacetate AM)
Cell Viability and Proliferation Assay Kit *Fluorimetric*
AnaSpec’s Calcein AM, a green fluorescent cell viability indicator, has excellent
quality, and is cost-effective.
1. Braut-Boucher, F. et al. J. Immunol. Methods 178, 41 (1995).
2. Akeson, AL. et al. J. Immunol. Methods 163, 181 (1993).
3. Weston , SA. et al. J. Immunol. Methods 133, 87 (1990).