Anti-TAU Antibodies – Industry’s largest selection


In Alzheimer's disease (AD), the major pathological hallmarks are β-sheet aggregates of β-amyloid peptides in senile plaques and hyperphosphorylated tau protein in neurofibrillary tangles (NFT).1-2

AnaSpec, a provider of one of the world's largest collections of β-amyloid peptides, also provides the industry's largest collection of anti-Tau antibodies - 23 phosphospecific and 14 non-phosphospecific Tau antibodies. Custom service for production of AD related peptides and antibodies is also available. For more information, please click here.

These Tau rabbit polyclonal antibodies, raised against Tau peptides, are supplied as epitope-affinity purified rabbit IgG at 25 or 50 μg sizes at 0.2 mg/ml of 1x PBS solution. They have been validated in dot blots and IHC (Figure 1). Species reactivity includes human, mouse and rat. Some antibodies have also shown reactivity with bovine, chicken and zebrafish. AnaSpec gratefully acknowledges Dr. Claudia Schwab for performing the Anti-Tau antibodies IHCs (in Dr. Patrick McGeer's lab, University of British Columbia, Vancouver, BC, Canada).

Tau is a collection of microtubule-associated proteins that is involved in microtubule assembly and stabilization.3 In adult human brain, 6 isoforms, ranging between 352 and 441 amino acids in length, are produced as a result of alternative RNA splicing.4-5 The expression of Tau isoforms is developmentally regulated, as only the smallest Tau polypeptide is expressed in the fetal brain. Hyperphosphorylated Tau is the major component of the paired helical filament of Alzheimer's disease. Anti-phospho-Tau antibodies are used to identify specific amino acids that are phosphorylated in Tau from normal brains and Alzheimer's disease brains. The Tau proteins, especially in developing brains and in Alzheimer brains, are phosphorylated in vivo at many different sites such as Thr181, Ser198, Ser199, Ser202, Thr205, Thr212, Ser214, Thr217, Thr231, Ser235, Ser262, Ser356, Ser396, Ser400, Ser404 and Ser413.6-7

 

Anti-Tau - Phospho & Non-Phospho Specific Antibodies


Complementing one of the world's most comprehensive collections of
β-amyloid peptides, the availability of these anti-Tau antibodies demonstrates AnaSpec's commitment to delivering integrated solutions for Alzheimer research.



Figure 1. IHCs of Anti-Tau, phosphorylated and non-phosphorylated antibodies. All antibodies strongly label neurofibrillary tangles (NFT), dystrophic neurites (DN) in neuropil and plaques, neuropil threads (NPT), and some glia cells in white matter in AD tissue (images courtesy of Dr. C. Schwab, Univ of British Columbia, Vancouver, Canada).

Figure 2. Dot blot of Tau antibodies.

Click here for more information.

Table 1. A full listing of AnaSpec's Tau phospho and non-phospho specific antibodies. All antibodies, except for the first item (cat# 53884), are polyclonal antibodies
.

Product

50 ug

25 ug

Anti-Tau

53884

NA

Anti-Tau (pThr181)

54960

54960-025

Anti-Tau (Thr181/Ser184)

54961

54961-025

Anti-Tau (pSer198)

54962

54962-025

Anti-Tau (pSer184)

55413

55413-025

Anti-Tau (pSer195)

55414

55414-025

Anti-Tau (Ser195)

55460

55460-025

Anti-Tau (pSer198)

54962

54962-025

Anti-Tau (pSer199/202)

54963

54963-025

Anti-Tau (pSer202)

28017

28017-025

Anti-Tau (Ser202)

28018

28018-025

Anti-Tau (pThr205)

54964

54964-025

Anti-Tau (194-214)

54965

54965-025

Anti-Tau (pThr212)

54966

54966-025

Anti-Tau (pSer214)

54967

54967-025

Anti-Tau (pThr217)

54968

54968-025

Anti-Tau (209-226)

54969

54969-025

Anti-Tau (pThr231)

55313

55313-025

Anti-Tau (Thr231)

55415

55415-025

Anti-Tau (pSer235)

55315

55315-025

Anti-Tau (Ser235)

55461

55461-025

Anti-Tau (pSer237)

55321

55321-025

Anti-Tau (Ser237)

55322

55322-025

Anti-Tau (pSer238)

55323

55323-025

Anti-Tau (pSer262)

54973

54973-025

Anti-Tau (Ser262)

54974

54974-025

Anti-Tau (pSer356)

54975

54975-025

Anti-Tau (Ser356)

54976

54976-025

Anti-Tau (pSer396)

54977

54977-025

Anti-Tau (pSer400)

54978

54978-025

Anti-Tau (pSer404)

28023

28023-025

Anti-Tau (Ser404)

28024

28024-025

Anti-Tau (393-411)

54979

54979-025

Anti-Tau (pSer409)

55417

55417-025

Anti-Tau (401-426)

55416

55416-025

Anti-Tau (pSer412)

55418

55418-025

Anti-Tau (pSer413)

55325

55325-025

 

Anti-Tau Antibody (Acetyl K280) & Anti-Tau Antibody (K280)

In support of AD and tauopathy research, AnaSpec is pleased to announce the release of two new polyclonal antibodies - Anti-Tau (Acetyl K280) and Anti-Tau (K280) antibodies. Acetylation of Tau at lysine 280 (K280) is a pathological modification in Tau-mediated neurodegeneration. This modification results in reduced solubility and microtubule assembly. It also increases Tau aggregation (2, 3).6-7 Studies have also shown that a single amino acid deletion (ΔK280) in tau protein promotes tau aggregation in vitro and in vivo.8-9

Anti-Tau Antibody (Acetyl K280)

Anti-Tau Antibody (K280)   

Tau antibody acetylK280 dot blot
 
Tau antibody acetylK280 WB
Tau antibody K280 dot blot
 
Tau antibody K280 WB
Fig. 1. Top: Dot blot of rabbit anti-Tau (Acetyl K280) polyclonal antibody using synthetic peptides: Tau (Acetyl K280), Tau (K280) dotted at 0.02, 0.2 and 1 µg. Bottom: WB of recombinant Tau proteins using rabbit anti-Tau (Acetyl K280) polyclonal antibody. M: Marker. Lane 1: recombinant Tau protein acetylated by p300 enzyme. Lane 2: recombinant Tau protein. Fig. 2. Top: Dot blot of rabbit anti-Tau (K280) polyclonal antibody using synthetic peptides: Tau (Acetyl K280), Tau (K280) dotted at 0.02, 0.2 and 1 µg. Bottom: WB of mouse brain lysate and recombinant Tau proteins using rabbit anti-Tau (K280) polyclonal antibody. M: Marker. Lane 1: mouse brain lysate. Lane 2: recombinant Tau protein acetylated by p300 enzyme. Lane 3: recombinant Tau protein.

Table 2. Anti-Tau rabbit polyclonal antibodies, Acetyl K280 and K280.

Product

Size

Catalog #

Anti-Tau Antibody (Acetyl K280) NEW

50 µg

Anti-Tau Antibody (K280) NEW

50 µg

56078

 
 
Anti-Tau Antibody (GlcNAc Ser400) & Anti-Tau Antibody (Ser400)

AnaSpec offers a polyclonal antibody that recognizes the glycosylated [N-acetyl-D-glucosamine (GlcNAc)] Serine400 residue. Anti-Tau Antibody (GlcNAc Ser400) was raised against a synthetic peptide derived from amino acids surrounding the Tau Ser400 sequence. As confirmed by Western and dot blots, this antibody only recognizes Tau glycosylated Ser400 and not the non-glycosylated Tau. Another polyclonal antibody, Anti-Tau (Ser400). This antibody recognizes all Tau modifications including phosphorylation and glycosylation.Species reactivity for both antibodies includes human, monkey, mouse, rat and other mammalian species.

Fig. 4. Recombinant Tau (2N4R) and rat brain lysate were probed with Anti-Tau (GlcNAc Ser 400) antibody. Secondary Goat Anti-Rabbit IgG-HiLyte™ Fluor 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used. Multiple immunoreactive bands were detected for rat brain lysate and none for recombinant Tau (as expected).
Fig. 5. Tau (GlcNAc Ser 400) and non-glycosylated immunizing tau peptides was probed with Anti-Tau (GlcNAc Ser 400) antibody. Secondary Goat Anti-Rabbit IgG-HiLyte™ Fluor 750 labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used. This antibody does not react with "naked" Tau peptide.
Fig. 6. Recombinant Tau (2N4R) and rat brain lysate were probed with Anti-Tau (Ser 400) antibody. Secondary Goat Anti-Rabbit IgG-HiLyte™ Fluor 750 nm labeled antibody (Cat# 61056-05-H750 or 61056-1-H750) was used to visualize. Multiple immunoreactive bands were detected for rat brain lysate and single band was detected for recombinant Tau (as expected).

Table 3. Anti-Tau rabbit polyclonal antibodies, GlcNAc Ser400 and Ser400.

Product

Size

Catalog #

Anti-Tau Antibody (GlcNAc Ser400) NEW

50 µg

Anti-Tau Antibody (Ser400) NEW

50 µg

55944

Related Product:

[Ser(OGlcNAc)]400-KWK-Tau (388-411)-KK(Biotin)-NH2 Peptide, 65409-025 (0.25 mg) & 65409-1 (1 mg)

Tau Peptides - 6 isoforms

Table 4. Additional Alzheimer's Disease related antibodies.

Product

Size

Catalog #

Anti - α - Synuclein (paired 87)

50 µg

55330

Anti - α - Synuclein (pSer 87)

25 µg

50 µg

55329-025

55329

Anti - β - Amyloid (1 - 40)

50 µg

54295

Anti - β - Amyloid (1 - 40), mouse monoclonal

50 µg

55921

Anti - β - Amyloid (1 - 42), mouse monoclonal

50 µg

55922

Anti - β - Amyloid (NT), human

50 µg

53224

Anti - APP (CT)
Amyoid beta A4 protein precursor, Alzheimer's disease amyloid protein

50 µg

54096

Anti - APP (paired668)

50 µg

54980

Anti - APP (pThr668)

25 µg

50 µg

54296-025

54296


Tau Recombinant Proteins

AnaSpec recently released two Tau-441 recombinant proteins, (with or without a GST tag). Tau-441 is the longest of the six Tau isoforms and contains 441 amino acids with molecular mass of 45.8 kDa. The sequence (Accession # AAC04279.1) in both proteins corresponds to the full length human Tau-441 (2N4R isoform) along with a GST tag. Protein was expressed in E.coli. For the protein without the GST tag, the tag was later cleaved and removed using Glutathione agarose.


Tau-441 recombinant protein
Fig. 6. Human Tau-441 on SDS-PAGE. Purified protein was loaded to a 10-20% Tris-HCl gel. CL=unpurified cell lysate, GST-Tau=purified GST-Tau fusion protein, Tau441=purified Tau without GST tag.
Fig. 7. Western blot of in vitro phosphorylated Tau-441 protein with human GSK-3β. Purified Tau-441 was incubated with human GSK-3β. Phospho-Tau-441 (+GSK) & unphospho-Tau-441(-GSK) were resolved on a 10-20% Tris gel & transferred to nitrocellulose membrane. Proteins were probed with anti-Tau (393-411) and phosphospecific anti-Tau(pSer400) antibodies (AnaSpec Cat #54979 & Cat#54978, respectively).
Tau-441 recombinant protein GST tagged
Fig. 8. Human GST-Tau-441 on SDS-PAGE. Purified protein was loaded onto 10-20% Tris-HCl gel. CL=unpurified cell lysate, GST-Tau=purified GST-Tau fusion protein.

Table 5. Tau-441 recombinant protein, with or without GST tag.

Product

Size

Catalog #

Recombinant Human Tau (Tau - 441) Protein NEW

50 µg
100 µg

55556-50
55556-100

Recombinant Human Tau (Tau - 441) Protein, GST tagged NEW

50 µg
100 µg

55557-50
55557-100

 

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Read more about Tau Products

References

1.Khachaturian, ZS. Arch. Neurol. 42, 1097 (1985).
2.Mirra, SS. et al. Neurol. 41, 479 (1991).
3.Cleveland, DW. et al. J. Mol. Biol. 116, 207 (1977).
4.Goedert, M. et al. Neuron 3, 519 (1989).
5.Geodert, M. et al. EMBO J. 8, 393 (1989).
6.Billingsley, M. et al. Biochem J. 323, 577 (1997).
7.Hanger, DP. et al. J. Neurochem. 71, 2465. (1998).