Myelin Oligodendrocyte Glycoprotein (MOG), a protein with a molecular
weight of 26- to 28-kDa, is a member of the immunoglobulin superfamily. It is
expressed exclusively in the central nervous system (CNS), specifically on the
surface of myelinating oligodendrocytes and external lamellae of myelin sheath.
MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins. However,
it has been demonstrated to be a crucial autoantigen for multiple sclerosis
(MS) in humans and experimental autoimmune encephalomyelitis (EAE) in rodents
and monkeys (1-6).
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These proteins, with sequences corresponding to human,
mouse and rat extracellular domain, together with a 6x His tag were expressed
in E. coli and purified from urea denatured bacterial lysate. The molecular
weight found in all 3 recombinant proteins was 14.23 kDa. These proteins were
shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for
Anti-MOG IgG ELISA Assay Kits
1. Linares, D. et al. Protein Expression and Purif. 34,
2. Bettadapura, J. et al. J. Neurochem. 70, 1593 (1998).
3. Oliver, AR. et al. J. Immunol. 171, 462 (2003).
4. Von Budingen, H-C. et al. J. Clin. Immunol. 21, 155 (2001).
5. Lyons, J-A. et al. Eur. J. Immunol. 29, 3432 (1999).
6. Von Budingen, H-C. et al. Eur. J. Immunol. 34, 2072 (2003).