AnaSpec, the world’s largest provider of on-demand GO®
peptides is pleased to offer a wide selection of Histone H1, H2A, H2B, H3 and
H4 peptides, especially the H3 peptides. The rapidly expanding Histone GO®
Peptides now include citrullinated sequences in addition to sequences that are
acetylated, mono-, di- or tri-methylated, phosphorylated and biotinylated.
Histone H1, H2A, H2B,
H3 & H4 Modifications (with or without biotin):
· Citrullinated
· Acetylated
· Methylated (mono, di, tri)
· Phosphorylated
Chromatin
exist either as the transcriptionally active euchromatin or as the transcriptionally
repressed heterchromatin state.1 Post-translational modifications
of histone “tails” (amino termini) have been implicated in the conversion between
the two states. 1-2 Covalent modifications, such as acetylation,
methylation, citrullination and phosphorylation, affect chromatin structure
and exquisitely regulate gene expression.3-6 In histones, citrullination
or deimination of arginine to citrulline (Cit), a non-conventional amino acid
in protein is mediated by peptidylarginine deiminase type 4 (PAD4 or PADI4)
in the presence of Ca2+; with PAD4 being the only PAD with
a nuclear localization.7-9 Cuthbert, et al. reported that PAD4 deiminates
unmodified arginine and monomethyl, but not dimethyl arginine.9 Wang,
et al. found that PAD4 demethyliminates
histones in vitro and in vivo, regulating both histone Arg methylation and gene
activity.10 Histone hypercitrullination catalyzed by PAD4 appears to play a critical
role in chromatin decondensation in granulocytes/neutrophils.11
Table 1. A listing of citrullinated histone peptides.
| Product |
Size |
Catalog
# |
| [Cit2/8/17]-Histone
H3 (1-21)-GGK(Biotin), amide
A-Cit-TKQTA-Cit-KSTGGKAP-Cit-KQLAGG-K(Biotin)-NH2 |
0.25 mg |
65404 |
| [Cit2,
pThr3]-Histone H3 (1-21)-GGK(Biotin)
A-Cit-pT-KQTARKSTGGKAPRKQLAGG-K(Biotin) |
0.25 mg |
65272-025
65272-1 |
| [Cit17]-Histone
H3 (1-21)-GGK(Biotin)
ARTKQTARKSTGGKAP-Cit-KQLAGG-K(Biotin) |
0.25 mg
1 mg |
65233-025
65233-1 |
| [Cit2]-Histone
H3 (1-21)-GGK(Biotin)
A-Cit-TKQTARKSTGGKAPRKQLAGG-K(Biotin) |
0.25 mg
1 mg |
65237-025
65237-1 |
| [Cit26]-Histone
H3 (21-44)-GGK(Biotin)
ATKAA-Cit-KSAPATGGVKKPHRYRPGGG-K(Biotin) |
0.25 mg |
65234-025
65234-1 |
| [Cit8]-Histone H3 (1-21)-GGK(Biotin)
ARTKQTA-Cit-KSTGGKAPRKQLAGG-K(Biotin) |
0.25 mg
1 mg |
65232-025
65232-1 |
| [Cit3]-Histone
H2A (1-22)-GK(Biotin)
SG-Cit-GKQGGKARAKAKSRSSRAGGG-K(Biotin) |
0.25 mg
1 mg |
65273-025
65273-1 |
| [Cit3]-Histone H4 (1-23)-GGK(Biotin)
SG-Cit-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin) |
0.25 mg
1 mg |
65275-025
65275-1 |
Click to view entire listing of Histone:
H1, H2A, H2B; H3 (1-21);
H3 (21-44);
H4 peptides.
Click the catalog numbers of the PDF listing to get to the online product page.
References:
1. Grunstein, M. et al. J Cell Sci Suppl 19, 29 (1995).
2. Strahl, BR and CD Allis, Nature 403, 41 (2000).
3. Strahl, BD. et al. Proc. Natl Acad Sci 96, 14967 (1999).
4. Jenuwein, T. and CD. Allis, Science 293, 1074 (2001).
5. Sterner, DE. et al. Microbiol Mol Biol Rev 64, 435 (2000).
6. Lachner, M. et al. J Cell Sci 116, 2117 (2003).
7. Vossenaar, ER. et al. Bioassays 25,
1106 (2003).
8. Nakashima, K. et al. J Biol Chem 277, 49562 (2002).
9. Cuthbert, GL. et al. Cell 118, 545 (2004).
10. Wang, Y. et al. Science 306, 279 (2004).
11. Wang, Y. et al. J Cell Bio 184, 205 (2009).
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