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| EnzoLyte™ Caspase Assay Kits - Optimized for HTS |
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EnzoLyte™ Caspase Assay Kits
Apoptosis, a programmed, cell-autonomous death process, plays important roles in a variety of physiological and pathological events1. These range from normal fetal development to diseases, such as cancer2, organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase3, DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C delta4. At least ten caspases have been discovered. Some of these caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate4.
AnaSpec is pleased to showcase its line of EnzoLyte™ Caspase Assay Kits. With features that include one-step homogeneous assays, a convenient mix-and-read format, and highly sensitive fluorimetric components, EnzoLyte™ Caspase Assay Kits are an ideal solution for high-throughput screening.
EnzoLyte™ Homogeneous Caspase-3/7 Assay Kits
- For detecting caspase 3/7 activity in cells and/or for screening caspase 3/7 inducers or inhibitors
- Convenient format: Measures activity directly in 96- or 384-well microplate, bifunctional lysis buffer lyses cells as well as provides optimal buffer condition for caspase 3/7 activity
EnzoLyte™ Homogeneous AFC Caspase-3/7 Assay Kit
500 assays in 96-well format **Industry's Best Value**
Ex/Em(nm)=370/500
EnzoLyte™ Homogeneous AMC Caspase-3/7 Assay Kit
500 assays in 96-well format **Industry's Best Value**
Ex/Em(nm)=340/440
EnzoLyte™ Homogeneous Rh110 Caspase-3/7 Assay Kit
500 assays in 96-well format **Industry's Best Value**
Ex/Em(nm)=501/527
EnzoLyte™ Caspase Profiling Kits
- For assaying caspase protease activities
EnzoLyte™ AMC Caspase Profiling Kit
8 AMC-based substrates coated to two 96-well plates, AMC fluorescence reference std coated on the same plate
Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Profiling Kit
8 AFC-based substrates coated to two 96-well plates, AFC fluorescence reference std coated on same plate
Ex/Em=370/500 nm
EnzoLyte™ Caspase Substrate Sampler Kits
- The best solution for identifying a suitable substrate for designing AFC-based caspase assays
EnzoLyte™ AMC Caspase Substrate Sampler Kit
8 AMC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Substrate Sampler Kit
8 AFC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
Ex/Em=370/500 nm
Related assay kits:
DHL™ Cell Cytotoxicity Assay Kit *Fluorimetric*
DHL™ Cell Viability and Proliferation Assay Kit *Fluorimetric*
To request AnaSpec’s latest Detection Reagents & Kits catalog, click here
References
- Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
- Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
- Lazebnik, YA. et al. Nature 371, 346-347 (1994).
- Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).
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