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5-Methylcytidine, mAb

AnaSpec, EGT Group is pleased to offer Eurogentec’s popular monoclonal antibody, anti-5-methylcytidine (Clone 33D3) for use in epigenetics research. Purified from ascites, this IgG1 / λ isotype antibody is supplied as a 1 mg/ml solution (in PBS) and is available in three sizes (0.1 mg, 0.5 mg and 1 mg).

5-Methylcytidine (5-MeCyd) is cytosine modified by methylation on the fifth position of the cytidine ring. This modification occurs naturally in the DNA of plants and vertebrate. DNA methylation is a post-replication process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Alterations of DNA methylation pattern are associated with carcinogenesis. A global DNA hypomethylation is often detected in tumor tissues, associated with local hypermethylation sites. Since this antibody discriminates between the modified base, 5-MeCyd and its normal counterpart, cytosine, it is a useful tool for gene promoter methylation analysis.

The most published anti-5-Methylcytidine antibody available on the market, anti-5-Methylcyidine from Clone 33D3 can be used in applications such as Methylated DNA Immunoprecipitation (MeDIP), MeDIP-on-chip assay, immunofluorescence, flow cytometry and immunohistochemistry. Histopathological analysis of tissue samples can be performed without having to destroy cells through DNA extraction procedures.

• Most cited anti-5-Methylcytidine antibody (Clone 33D3)
• Epigenetic research tool
• For use in applications such as
— Methylated DNA Immunoprecipitation (MeDIP)
— MeDIP-on-chip assay
— Flow cytometry
— Immunohistochemistry

This antibody has been used to detect alterations in the urinary excretion of nucleosides from cancer patients, to visualize the distribution of methyl-rich regions along human chromosomes, to quantify in situ differences between normal and malignant cells from peripheral blood as well as on tissue sections.

Figure 1. 5-Methylcytidine (5mC) was detected in HeLa cells with anti-5mC at 1:125 dilution and visualized with HiLyte Fluor™ 555 labeled goat anti-mouse IgG (Cat# 61057-05-H555), in red. α-Tubulin was detected with anti-α-tubulin and visualized with FITC-labeled goat anti-rabbit IgG (Cat# 28176-05-FITC), in green.

Product	Size	Catalog #
Monoclonal Antibody against 5-Methylcytidine Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics	0.1 mg	BI-MECY-0100
Monoclonal Antibody against 5-Methylcytidine Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics	0.5 mg	BI-MECY-0500
Monoclonal Antibody against 5-Methylcytidine Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics	1 mg	BI-MECY-1000

The following papers are examples of recent publications citing the use of this antibody. To view a citation archive, please click here.

Tao, Y. et al. (2011). Lsh, chromatin remodeling family member, modulates genome-wide cytosine methylation patterns at nonrepeat sequences. PNAS 108, 5626.

Monk, D. et al. (2011). Human imprinted retrogenes exhibit non-canonical imprint chromatin signatures and reside in non-imprinted host genes. Nucleic Acid Res. doi:10.1093/nar/gkq1230.

Iqbal, K. et al. (2011). Reprogramming of the paternal genome upon fertilization involves genome-wide oxidation of 5-methylcytosine. PNAS 108, 3642.

Jin, SG. et al. (2010). Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine. Nucleic Acid Res. doi:10.1093/nar/gkq22.

Sanchez-Abarca, LI. et al. (2010). Immunomodulatory effect of 5-azacytidine (5-azaC): potential role in the transplantation setting. Blood 115: 107, doi: 10.1182/blood-2009-03-210393.

Other epigenetics products include a wide selection of histone peptides, especially histone H3 peptides, SensoLyte^® HDAC, LSD1 and SIRT1 & 2 assay kits.