Custom Peptide Synthesis


Since our inception in 1993, AnaSpec has assisted scientists in the design and synthesis of >50,000 peptides. As a result, our combined knowledge and expertise provide premium solutions to our customers.

AnaSpec routinely completes difficult peptide synthesis such as synthesis of long peptides (>90-mers), peptides containing unusual modifications, and cyclic peptides. In addition, we have the capacity for large-scale synthesis (>100 g).

Optimized Fmoc and Boc methodologies are employed for peptide syntheses. Cleavage of peptides is performed using trifluoroacetic acid (TFA), hydrogen fluoride (HF), or other appropriate methods. Most of the peptides are purified by HPLC using reverse phase C4 and C18 columns. Alternative purification methods (e.g., ion exchange and gel filtration chromatography) are used when needed. AnaSpec is equipped with state-of-the-art automated peptide synthesizers capable of performing synthesis ranging from small (0.025 mmol) to large (50 mmol) scale. These state-of-the-art synthesizers, coupled with our experienced peptide chemists, enable us to deliver our peptides quickly and in high quality (purity of 90-95% is typical). Most custom orders can be expected to be delivered in two to three weeks. Complete confidentiality of all orders is assured.

AnaSpec’s synthesis facilities located in Fremont, California means that we are able to provide comprehensive oversight of the entire peptide synthesis process. This ensures that we consistently meet our customer’s product specifications. All custom peptides are accompanied with MS and HPLC analyses. Additional analytical data (e.g., amino acid analysis, sequencing) are available upon request. AnaSpec is also proud to be certified to ISO 9001:2008.

Custom Peptide Modifications

• Alkyne peptides
• Azide peptides
• BSA, KLH conjugated peptides for antibody production

• C-terminal modifications

* Aldehydes

* Alcohols

* Chloromethyl Ketones

* Cysteamide

* Ester

* N-Alkyl Amides

* Hydrazides

* Thio ester

• Cyclic peptides

* N->C

* Disulfide (S-S bond formation)

• Dehydro peptides

* dehydroalanine; dehydroleucine, dehydroproline

• Dityrosine Cross linked peptides

• Drug-peptide conjugates

• Dye-labeled peptides:

* C-terminal

* AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110

* N-terminal

* HiLyte™ Flour dyes (405, 488, 555, 647, 680, 750)

* QXL™ quenchers

* FAM, FITC, MCA, Rox, Sulforhodamine 101, TAMRA

• FRET Substrates

* 5-FAM/QXL™ 520

* HiLyte™ Fluor 488/QXL™ 520

* TAMRA/QXL™ 570

* EDANS/DABCYL and other FRET pairs

• Glycopeptides

• Heavy Isotope Labeled Peptides (ClearPoint peptides labeled with D, C13, N15)

• Hydrocarbon Stapled Peptides

• Lactam peptides

• Lipopeptides

* Prenylated

* Farnesyl

* GeranylGeranyl

• MAPS (Multiple Antigenic Peptide)

• Methylated peptides

* Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, Arg(Me)2 assymmetrical

* Na-Methylated

* Ca-Methylated

• N-terminal Modifications

* Acetylated

* Biotinylated

* Bromoacetylated

* DOTA

* Formylated

* Myristoylated

* Succinylated

• Pegylated peptides

• Peptide libraries

* 96-well plate libraries

* One-Bead One-Peptide

• Peptidomimetics

• Peptide bond modifications

* Ester (Depsipeptides)

* Amine (reduced amide bond)

• Phosphopeptides

* Phosphoserine, Phosphothreonine, Phosphotyrosine

• Sulfated Tyrosine or Serine

• Thiolactone peptide

• TR-FRET Substrates

* Europium/QXL™ 610

* Europium/HiLyte™ Fluor 647

• Unusual Synthesis

For additional information about our custom peptide synthesis services, please contact service@anaspec.com

For online custom peptide synthesis quote request, please click here, or contact quotes@anaspec.com.