When traditional protocols can take almost 90 days, the wait for high-quality custom polyclonal antibodies can be excruciating. AnaSpec asked, "Is there a way to develop custom antibodies faster without sacrificing quality in the finished product?" Here's the answer.

AnaSpec is pleased to introduce the Speedy 28-day custom polyclonal antibody service. Leveraging proven technology from Eurogentec, AnaSpec's parent company, this protocol uses a proprietary mixture of Freund's free immuno-stimulatory compounds. Antibodies can be raised against peptide sequences consisting of standard, phosphorylated, methylated or acetylated amino acids. The first bleed is obtained at Day 21 after 4 immunizations. Bleed is guaranteed to have a titer of at least 1/20,000 for one of the immunized rabbits.

Figure 1. Comparison data between the Speedy 28-day and 87-day protocols. A KLH-conjugated peptide was used in raising antibodies in both protocols and titer based on ELISA was compared.

Pricing of this polyclonal antibody package includes:

• Peptide design from protein of interest
• Peptide synthesis (>80%, 15 aa, add 3-4 weeks)
  Standard peptide sequence
  Phosphorylated peptide sequence
  Methylated peptide sequence
  Acetylated peptide sequence

• Conjugation to carrier protein (KLH, BSA or OVA)
• Choice of two New Zealand rabbits in SPF or non-SPF environment
• 28-day immunization protocol (not including peptide synthesis)
• ELISA at Day 28
• Antigens include single peptide or two peptides or customer-supplied protein (titer guarantee does not apply to customer-supplied protein)

To start your 28-day immunization polyclonal project, contact service@anaspec.com to obtain a price quotation.

Related Antibody Custom Services:

Custom Polyclonal Antibody Production
Custom Hybridoma Development and Monoclonal Antibody Production
Peptide-Carrier Protein Conjugation
Antibody Purification and Labeling