AnaSpec’s SensoLyte^® MMP assay kits, especially the SensoLyte^® 520 MMP Assay Kits, have become the new standard in MMP assay sensitivity. With published results in world-class journals and the industry’s longest wavelength, the SensoLyte^® 520 line is one of the most sensitive (Figure 1) and brightest lines of MMP assay kits in the world.

SensoLyte^® 520 MMP Assays

• Measure MMP activity
• 96-well or 384-well format
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence
• Assay time – as short as 1h

Figure 1. The performance of an identical peptide sequence, one coupled with 5-FAM/QXL^TM 520 and the other coupled with EDANS/DABCYL FRET pairs was compared (A). Fluorescence was measured after 1 hr incubation of FRET substrates with serial dilutions of MMP-13 (Flex800, Bio-Tek instruments). A 5-FAM/QXL^TM 520 MMP substrate was compared with Mca/Dpa FRET substrate (B). Fluorescence was measured after 1 hr incubation of substrates using serial dilutions of MMP-13 (Flexstation 384II, Molecular Devices).

Table 1. A listing of the MMP assay kits and recombinant MMPs

SensoLyte^® Plus 520 MMP Assays

• Measure MMP activity
• Enhanced specificity – human capture antibody included
• 96-well format (strips)
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence