AnaSpec, EGT Group is pleased to announce the release
of an ultrasensitive ELISA assay for quantitatingα-synuclein.
The SensoLyte® α-Synuclein
Quantitative ELISA Kit (Human/Mouse/Rat) provides a convenient and quantitative
assay for determining α-Synuclein amount in cell and tissue lysate as well
as in body fluids. Compared to other anti-α-Synuclein ELISA kits on the market,
this one-step, mix-and-read assay takes less time to run. HRP conjugated detection
antibody is added simultaneously with the samples and standards during the assay,
eliminating extra incubation and washing steps.
| Product |
Catalog
# |
| SensoLyte® Anti-α-Synuclein
(Human, Mouse, Rat) ELISA Kit |
55550 |
§ One Step Convenient Format
* Pre-coated and pre-blocked 96-well strip plate
* Ready-to-use substrate solution and other assay components
* One step assay (samples and detection antibody are added simultaneously)
* 1 hour assay time at room temperature (excluding incubation time)
§ Minimal Sample Size
* Requires only 10-20 μl of cell and tissue lysate or body fluids
§ High Sensitivity
* Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)
§ Broad Dynamic Range
* 8-500 pg α-Synuclein/ml in the assayed sample
Figure 1. An example of the α-Synuclein ELISA standard curve
α-Synuclein is a major
component of Lewy bodies found in affected neurons of patients with Parkinson’s
disease.1-4 This protein has a mass of 14.5 kDa (140 amino acids
long) and consists of a conserved degenerative amino-terminal domain and an
acidic carboxyl-terminal that has a higher sequence divergence.1-4
α-Synuclein is predominantly expressed in brain, specifically in cerebellum,
thalamus, neocortex, hippocampus, and striatum regions.1-2 Other
tissues also express α-Synuclein but at very low levels.1-4
The physiological role of α-Synuclein is not well understood. However,
the presence of imperfect KTKEGV lipid interacting repeats suggests that it
may be involved in synaptic vesicle homeostasis.3
Related Products
Table 1. A listing of Synuclein recombinant proteins
| Product |
Size |
Catalog
# |
| Recombinant human α - Synuclein (1 - 140) |
500 µg
1000 µg |
55456-500
55456-1000 |
| Recombinant human α - Synuclein (1 - 140), biotin
labeled |
200 µg |
55581 |
| Recombinant human α - Synuclein (1 - 140), HiLyte
Fluor™ 488 labeled |
200 µg |
55457 |
| Recombinant Human β - Synuclein (1 - 134) |
100 µg
500 µg
1000 µg |
55458-100
55458-500
55458-1000 |
Table 1. A listing of Synuclein antibodies.
| Product |
Size |
Catalog
# |
Anti-α-Synuclein (mouse) |
50 µg |
53877 |
| Anti-α-Synuclein (pSer 87)
|
25 µg
50 µg |
55329-025
55329 |
| Anti-α-Synuclein (paired 87) |
50 µg |
55330 |
| Anti-Synuclein (pan)(IN) |
50 µg |
55916 |
| Anti-ß-Synuclein |
50 µg |
53879 |
References:
- Rivers, R. et al. Protein Science 17, 887 (2008).
- Bruening, W. et al. Cancer 88, 2154 (2000).
- George M. J. Genome Biology3, 1 (2001).
- Latawiec, D. et al. PloS ONE 5, 1 (2010).
|
