SensoLyte® α-Synuclein ELISA Assay – Ultrasensitive


AnaSpec, EGT Group is pleased to announce the release of an ultrasensitive ELISA assay for quantitatingα-synuclein. The SensoLyte® α-Synuclein Quantitative ELISA Kit (Human/Mouse/Rat) provides a convenient and quantitative assay for determining α-Synuclein amount in cell and tissue lysate as well as in body fluids. Compared to other anti-α-Synuclein ELISA kits on the market, this one-step, mix-and-read assay takes less time to run. HRP conjugated detection antibody is added simultaneously with the samples and standards during the assay, eliminating extra incubation and washing steps.

Product

Catalog #

SensoLyte® Anti-α-Synuclein (Human, Mouse, Rat) ELISA Kit

55550







§
One Step Convenient Format
* Pre-coated and pre-blocked 96-well strip plate
* Ready-to-use substrate solution and other assay components
* One step assay (samples and detection antibody are added simultaneously)
* 1 hour assay time at room temperature (excluding incubation time)

§ Minimal Sample Size
* Requires only 10-20 μl of cell and tissue lysate or body fluids

§ High Sensitivity
* Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)

§ Broad Dynamic Range
* 8-500 pg α-Synuclein/ml in the assayed sample




Figure 1. An example of the α-Synuclein ELISA standard curve

α-Synuclein is a major component of Lewy bodies found in affected neurons of patients with Parkinson’s disease.1-4 This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal that has a higher sequence divergence.1-4 α-Synuclein is predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions.1-2 Other tissues also express α-Synuclein but at very low levels.1-4 The physiological role of α-Synuclein is not well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.3


Related Products

Table 1. A listing of Synuclein recombinant proteins

Product

Size

Catalog #

Recombinant human α - Synuclein (1 - 140)

500 µg

1000 µg

55456-500

55456-1000

Recombinant human α - Synuclein (1 - 140), biotin labeled

200 µg

55581

Recombinant human α - Synuclein (1 - 140), HiLyte™ Fluor 488 labeled

200 µg

55457

Recombinant Human β - Synuclein (1 - 134)

100 µg

500 µg

1000 µg

55458-100

55458-500

55458-1000



Table 1. A listing of Synuclein antibodies.

Product

Size

Catalog #

Anti-α-Synuclein (mouse)

50 µg

53877

Anti-α-Synuclein (pSer 87)

25 µg

50 µg

55329-025

55329

Anti-α-Synuclein (paired 87)

50 µg

55330

Anti-Synuclein (pan)(IN)

50 µg

55916

Anti-ß-Synuclein

50 µg

53879













References:

  1. Rivers, R. et al. Protein Science 17, 887 (2008).
  2. Bruening, W. et al. Cancer 88, 2154 (2000).
  3. George M. J. Genome Biology3, 1 (2001).
  4. Latawiec, D. et al. PloS ONE 5, 1 (2010).