AnaSpec,
the provider of the ultra-sensitive SensoLyte® 520 Human Renin
Assay Kit now provides renin kits specific for mouse and rat - the
SensoLyte® 520 Mouse
Renin Assay Kit and SensoLyte® 520 Rat
Renin Assay Kit. Using our proprietary 5-FAM/QXL™ 520 fluorescence resonance
energy transfer (FRET) peptide, all three assays are ideal for screening of
renin inhibitors and for continuous assay of renin activity, especially for
high throughput screening (HTS) purposes. In the intact FRET peptide, the fluorescence
of 5-FAM is quenched by QXL™ 520. However, upon cleavage into two separate fragments
by rat renin, the fluorescence of 5-FAM is recovered, and can be monitored at
excitation/emission = 490/520 nm. With a high fluorescence quantum yield and
long emission wavelength, the signal of 5-FAM can be detected with less interference
from the autofluorescence of cell components and test compounds. The assays
are performed in a convenient 96-well microplate format and can be adjusted
to 384-well microplate format.
• Ultra-sensitive assays
• FRET based substrates
• Long wavelength
• Minimal autofluorescence
• Renin assays kits specific for human, mouse & rat
The renin–angiotensin system (RAS) plays a central role in the
regulation of blood pressure and electrolyte homoeostasis.1 At the
first and rate-limiting step of the RAS cascade, renin, a highly specific aspartyl
protease, cleaves angiotensinogen, produced in the liver, to yield angiotensin
I, which is further converted into angiotensin II by ACE (Angiotensin Converting
Enzyme). Angiotensin II constricts blood vessels leading to increased
blood pressure. It also increases the secretion of ADH and aldosterone, and
stimulates the hypothalamus to activate the thirst reflex. Since an overactive
renin-angiotensin system leads to hypertension, renin is an attractive target
for the treatment of this disease. 2-4
Figure 1. Comparison data of 3 renin assay kits. SensoLyte® 520
Human Renin Assay Kit (Cat# 72040), SensoLyte® 390 Renin Assay Kit (Cat# 72039) and
a kit from company B.
Figure 2. A schematic representation of the cleavage of
a FRET peptide substrate by renin is shown on top. The absorption spectrum
of QXL™ 520 perfectly overlaps with the emission spectrum of 5-FAM (bottom).
Related Products
Human
Renin, Recombinant (5ug)
Mouse
Prorenin, Recombinant (20 ug)
Rat
Renin, Recombinant (20 ug)
SensoLyte®
390 Renin Assay Kit
SensoLyte®
390 ACE2 Activity Assay Kit
Angiotensins
and Related Peptides
Natriuretic
Peptides (ANP, BNP & CNP)
References:
1. He, FJ. and GA. MacGregor, J.
Renin Angiotensin Aldosterone Syst. 4, 11 (2003).
2. Wood, JM. et al., Hypertension, 7,
797 (1985).
3. Shibasaki, M. et al., Am. J. Hypertens.
4, 932 (1991).
4.
Wood,
JM.et al., Biochem. Biophys. Res. Comm. 308, 698 (2003).
|
