Peptide-7-amino-4-methylcoumarin conjugates (peptide-AMCs)
are widely used as fluorogenic substrates to study proteases. The preparation
of peptide-AMCs usually involves solution phase synthesis since AMC lacks an
additional functional group for attachment to a solid support. Although solid-phase
peptide synthesis (SPPS) of peptide-7-amino-4-carbamoylmethylcoumarin conjugates
(peptide-ACCs) as an alternative for peptide-AMCs has been previously reported;1,2 no AMC solid support resin for SPPS has ever been reported.
AnaSpec, EGT Group is pleased to provide a novel solid support AMC resin for facile solid-phase synthesis of peptide-AMCs.
Fmoc-ACA-Wang resin, AMC resins preloaded with Asp (D) and Arg (R) for use in
SPPS, as well as Fmoc-ACA-OH are now available from AnaSpec.
to request information on AMC resins pre-loaded with other amino acids and for bulk purchases.
Harris, JL. et al. Proc. Natl. Acad. Sci. USA 97,
Maly, DJ. et al. J. Org. Chem. 67, 910 (2002).