Large Scale Antibody Production
Printable Version Contact proteomics.services@eurogentec.com

Large Scale Antibody Project
Polyclonals & Monoclonals
Flexible Customization
For over 25 years we have been
a leading supplier of custom
antibodies to IVD and biotech
partners worldwide. We have
acquired a strong expertise
in developing both custom
monoclonal and polyclonal
antibodies in various hosts.
We are able to handle large
demanding projects with
remarkable flexibility to meet
the customer's requirements
and SOP's. A project manager
assigned to every project
ensures that your request will be
considered in a professional way.
25 YEARS OF EXPERTISE
IN CUSTOM ANTIBODY
DEVELOPMENT AS A GUARANTEE.
■ Polyclonal Antibody Production

Polyclonal antibodies are produced in different hosts including SPF animals. Projects of > 10 000 rabbits can be handled.

You can use your own antigen or we can synthesize peptide(s) for immunization. We have been designing peptides for immunization for over 20 years and have developed specific algorithms for the most optimal epitope design. Accordingly our success rate is > 95%.

We can manage classical projects as well as those that are more demanding, including BSL 2 antigens. Immunization scheme can follow our proprietary Speedy 28-day program or classical 87-day program, or be adapted to the customer's own program.

We have a high expertise in producing antibodies against Posttranslational modifications. By far the most popular request for PTM is Anti-Phosphorylation but we also offer Anti-Methylation, Anti-Acetylation and many more.

■ Monoclonal Antibody Development and Production

We generate monoclonal antibodies from hybridoma's using standard methods, and we are particularly experienced in producing mAbs from hybridoma's developed in house, or provided by the customer.

Monoclonal antibodies are produced in vitro in accordance with Belgian animal regulations and the 2010-63-UE directive for animal welfare. Based on the requested antibody amount and the clone productivity, we select the best production scheme, using flasks, roller bottles or bioreactors.

Our facilities are adapted to produce grams of mAbs. For requests of ≥ 200mg, a test study is systematically performed to determine the clone productivity. The cost of the test study is deducted from the production batch cost.



Species Mean Housing Capacity
Rabbits 15000*
Guinea Pigs 300*
Hamsters 200*
Rats 200*
Mice 400*
Can be easily adapted to larger customer's requests.
Regulation

Our Quality Management System is ISO9001 certified for the development, production and sale of products and services for research and development in Life Science.
Our animal facilities comply with the most stringent association's requirements, including:

  • UK Home Office Animals Scientific Procedures Act
    (The UK law carefully regulates the use of animals in scientific research and is amongst the most comprehensive and rigorous standards in the world)
  • Federation of European Laboratory animal Science Associations (FELASA)

Located in Belgium, our facilities are recognized by the authorities who operate under Belgium and EU Law. Our accreditation is global, and covers our facilities, our animal care system, and the high level of our staff training.

Each animal is selected with the highest vigilance and identified to guarantee traceability. Animal sources are strictly controlled, and statistical data regarding the use of animals are recorded. An internal Ethical Committee (IACUC) is in place. The Ethical Committee plays a strategic role in evaluating each new protocol and carrying out reviews every 5 years.

National Authorities regularly inspect our facilities for compliance regarding animal origin, identification, housing and welfare. In addition to numerous exchanges and feedback from our customers, this makes our animal housing one of the most cleanest and safest in the world.

Animal Welfare & Facilities

Animal Welfare is our major concern. We have strict rules and maintain a constant technological watch to comply with the highest standards for animal protection. Accordingly animal housing and immunizations are centered on the 3R's guidelines (Replacement, Reduction and Refinement). Our qualified staffs follow continuous training to stay at the highest level regarding animal welfare and care. The whole staff experience is registered in personal training files including education, experience, skills and training information.

Our Animal facilities are governed by stringent practices:

  • Facilities are cleaned 3-5 times a week with cleaning agents free from formaldehyde.
  • Flow of traffic from the cleanest to the dirtiest area prevents cross-contamination.
  • Daily observation of sentinel animals and health status are monitored as per FELASA recommendations.
  • Each Animal has a balanced diet and the feed is quality controlled.
  • Temperature, pressure, relative humidity and ventilation systems (100 % HEPA filtered air for SPF animals) are recorded in real time.
  • 24 hour staffed facilities with an intrusion alarm system, armored doors and surveillance camera ensure absolute security.

At any time, experts can visit our facilities and audits can be organized.

■ Confidentiality

All monoclonal and polyclonal immunization projects are confidential. All obtained hybridoma's, sera and results are and remain the property of the customer. Eurogentec guarantees not to claim any rights on the hybridoma or the antibodies. Non-disclosure agreements can be executed.




Contact proteomics.services@eurogentec.com

AnaSpec EGT, based in Fremont California, has assisted researchers with their epitope selection and custom antibody productions since 2000. We specialize in the generation of antibodies against peptide antigens to post-translational modification (PTM) targets, such as acetylation, methylation, and phosphorylation.

We offer both custom monoclonal and custom polyclonal antibody production service in a variety of species, including chicken, rabbit, and goat. Choose our 70-day, or our 28-day fast polyclonal antibody (pAb) service. We have the experience you can trust and complete confidentiality of all orders is assured. We can accommodate your need for AAALACi certification and OLAW assurance.

Order by Quotation Number - if you have requested and received a quotation before, and would like to order by the quotation number, please click here.

For additional information about our custom antibody production services, please contact
service@anaspec.com.

Order / Quotation Form
 
Antibody Project Name:
Target Protein Name:
Sequence:
Antibody Application:
(ie: WB, IHC, IP, CHIP, etc)
Special Requirements:
Select One Option to Submit Quote Request:




Animal Type (Select One Option)
Antigen Information for Epitope Analysis
:
Special Request
Antibody Purification (Select One Option)
Antibody Labeling (Select One Option)
Quantity of labeling (Select One Option):








Animal Type (Select One Option)
Antigen Information for Epitope Analysis
:
Special Request
Antibody Purification (Select One Option)
Antibody Labeling (Select One Option)
Quantity of labeling (Select One Option):









Animal Type (Select One Option)
Antigen Information for Epitope Analysis
:
Special Request
Antibody Purification (Select One Option)
Antibody Labeling (Select One Option)
Quantity of labeling (Select One Option):







Special Request
Antibody Purification (Select One Option)
Antibody Labeling (Select One Option)
Quantity of labeling (Select One Option):

Hybridoma Clone Origin (Animal Species)

*Mycoplasma positive cell lines cannot be accepted.
Hybridoma Clone Name (ID)
Hybridoma Clone Growth Rate
Antibody Propagation Method
Antibody Production Scale
mg
Antibody Purification
Antibody Labeling
Quantity of labeling:







  Alanine
Arginine
Asparagine
Aspartic Acid
Cysteine
Glutamic Acid
Glutamine
Ala (A)
Arg (R)
Asn (N)
Asp (D)
Cys (C)
Glu (E)
Gln (Q)
  Glycine
Histidine
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine
Gly (G)
His (H)
Ile (I)
Leu (L)
Lys (K)
Met (M)
Phe (F)
  Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine
Pro (P)
Ser (S)
Thr (T)
Trp (W)
Tyr (Y)
Val (V)