Peptide Synthesis: Custom peptide synthesis services from AnaSpec

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Custom Peptide Synthesis

AnaSpec provides custom peptides to customers worldwide, including researchers at universities and in biotechnology and pharmaceutical companies. Since our inception in 1993, we have assisted researchers in the design and synthesis of >30,000 peptides. As a result, when assisting you with your peptide needs, we have years of experience to draw from to provide you with optimal solutions.

AnaSpec routinely completes difficult peptide synthesis such as synthesis of long peptides (>80-mers), peptides containing unusual modifications, and cyclic peptides. In addition, AnaSpec has the capacity for large-scale synthesis (>1 kg). Optimized Fmoc and Boc methodologies are employed for peptide syntheses. Cleavage of peptides is performed using trifluoroacetic acid (TFA), hydrogen fluoride (HF), or other appropriate methods. Most of the peptides are purified by HPLC using reverse phase C4 and C18 columns. Alternative purification methods (e.g., ion exchange and gel filtration chromatography) are used when needed. AnaSpec is equipped with state-of-the-art automated peptide synthesizers capable of performing synthesis ranging from small (0.025 mmol) to large (50 mmol) scale. These state-of-the-art synthesizers, coupled with our experienced peptide chemists, enable us to deliver our peptides quickly and in high quality (purity of 90-95% is typical). Most custom orders can be expected to be delivered in two to three weeks. Complete confidentiality of all orders is assured.

Because AnaSpec maintains its own facilities located in Fremont, CA, we are able to provide comprehensive oversight to ensure the highest standards of quality. AnaSpec is proud to be an ISO 2001 certified facility. All custom peptides are accompanied with MS and HPLC analyses. Additional analytical data (e.g., sequencing, amino acid analysis) are available upon request.

AnaSpec's Total Quality Management (TQM) Advantage

GMP Peptides

Custom Peptide Modifications

BSA, KLH conjugated peptides for antibody production
Click Chemistry peptides
C-terminal modifications

Aldehydes
Alcohols
Chloromethyl Ketones
Cysteamide
Ester
N-Alkyl Amides
Hydrazides
Thio ester

Cyclic peptides

N -> C
Disulfide (S-S bond formation)

Dehydro peptides

dehydroalanine; dehydroleucine, dehydroproline

Dityrosine Cross linked peptides
Drug-peptide conjugates
Dye-labeled peptides:

C-terminal

AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110

N-terminal

HiLyte Flour™ dyes (405, 488, 555, 647, 680, 750)
QXL™ quenchers
FAM, FITC, MCA, Rox, Sulforhodamine 101, TAMRA

FRET Substrates

5-FAM/QXL-520
HiLyte Fluor 488/QXL-520
TAMRA/QXL-570
EDANS/DABCYL and other FRET pairs

Glycopeptides
Heavy Isotope Labeled Peptides (ClearPoint peptides labeled with D, C13, N15)
Hydrocarbon Stapled Peptides
Lactam peptides
Lipopeptides

Prenylated
Farnesyl
GeranylGeranyl

MAPS (Multiple Antigenic Peptide)
Methylated peptides

Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, Arg(Me)2 assymmetrical
N^a-Methylated
C^a-Methylated

N-terminal Modifications

Acetylated
Biotinylated
Bromoacetylated
DOTA
Formylated
Myristoylated
Succinylated

Pegylated peptides
Peptide libraries

96-well plate libraries
One-Bead One-Peptide

Peptidomimetics
Peptide bond modifications

Ester (Depsipeptides)
Amine (reduced amide bond)

Phosphopeptides

Phosphoserine, Phosphothreonine, Phosphotyrosine

Sulfated Tyrosine or Serine
Thiolactone peptide
TR-FRET Substrates

Europium/QXL-610
Europium/HiLyte Fluor 647

Unusual Synthesis

For additional information about our custom peptide synthesis services, please contact service@anaspec.com.

Order / Quotation Form

For entering sequences, please use three-letter amino acid codes starting with the N-terminus and ending with the C-terminus; the ends of the sequence should be labeled with H- for N-terminal amine and -OH or -NH2 for C-terminal acid or amide. For substitutions on the peptide, please use standard chemical abbreviations provided.

Order by Quotation Number - if you have requested and received a quotation before, and would like to order by the quotation number, please click here.

Chemical Abbreviations
Technical Notes

Alanine
Arginine
Asparagine
Aspartic Acid
Cysteine
Glutamic Acid
Glutamine

Ala (A)
Arg (R)
Asn (N)
Asp (D)
Cys (C)
Glu (E)
Gln (Q)

Glycine
Histidine
Isoleucine
Leucine
Lysine
Methionine
Phenylalanine

Gly (G)
His (H)
Ile (I)
Leu (L)
Lys (K)
Met (M)
Phe (F)

Proline
Serine
Threonine
Tryptophan
Tyrosine
Valine

Pro (P)
Ser (S)
Thr (T)
Trp (W)
Tyr (Y)
Val (V)

Peptide Reference # (Optional):			Number of Amino Acids:
Sequence:

Requirements:

----- N-Term -----
Free Amine
Acetyl
Biotin
Biotin-LC
Other:

----- C-Term -----
Free Acid
Amide
Other:

----- Purity -----
Unpurified
>70%
>80%
>90%
>95%
>98%

----- Quantity -----
5 mg
10 mg
20 mg
50 mg
100 mg
Other:

Conjugation:		mg
Carrier:		KLH BSA


	Yes	No
Polyclonal Anti-Peptide Antibodies?
S-S Bond Formation?
Amino Acid Analysis?
GMP Grade?

Literature Citations

"...addition of 1 mg/mL RGD-4C (AnaSpec). Analysis of Peptides for Specificity and Cross-Reactivity...coverslips. Binding of Peptide-Coated Microparticles to...Tumor Cells Synthetic cyclic peptides (AnaSpec) were modified through the..."
Dane, KY. et al. Mol. Cancer Ther. 8, 1312 (2009).

"...binding site, synthetic peptides were synthesized by AnaSpec, San Jose, CA and... Synthetic peptides were purchased from AnaSpec, San Jose, CA. Horseradish...binding site, synthetic peptides were synthesized by AnaSpec, San Jose, CA. Six..."
Coffman, LG. et al. PNAS 106, 570 (2009).

"...Assays and Analysis of Synthetic Peptides-The enzymatic activity of EP24...based on analysis of products, peptides were selected and custom-synthesized (AnaSpec Inc, San Jose, CA). In these assay, 100 mum of each peptide was incubated with 2.5 nm purified..."
Berti, DA. et al. J. Biol. Chem. 284, 14105 (2009).

"...an Applied Biosystems model 433A peptide synthesizer using standard FastMoc...chemistry (22). Synthesis of biotinylated peptides was carried out with Fmoc-Lys (Biotin-LC)-Wang resin (AnaSpec). The crude peptides were precipitated, washed with butyl..."
Zhang, P. et al. PNAS 106, 7537 (2009).

"...Peptides were synthesized to our specifications (AnaSpec)..."
Mintz, PJ. et al. PNAS 106, 2182 (2009).

"...overnight with one of 43 biotinylated peptides (AnaSpec, San Jose, CA) at 12.5 um..."
Berrodin, TJ. et al. Mol. Endocrinol. 23, 74 (2009).

"...(ASNENMETM) were used as negative controls. All peptides were synthesized by AnaSpec, Inc. (San Jose, CA)..."
Ruckwardt, TJ. et al. J. Virol. 83, 3019 (2009).