IMG-EN-AS-72017-01.jpg
Assay-kits

SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

$663.00
Check your price
  • Cat.Number : AS-72017
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

Alternative choices

Quantity

Add to wishlist Bulk Quote

The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-9 antibody: 12 X 8 black strips Component B: MMP-9 standard, recombinant human proMMP-9: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 5 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-9 substrate, 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffe:r 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P14780
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component B at -80 °C. Store all other components at -20 °C. Components D, G, H and I can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

Downloads

You may also be interested in the following product(s)

IMG-EN-AS-60431-1-01.jpg

ADAMTS-4/Aggrecanase FRET Substrate, WAAG-3R - 1 mg

Cat.Number : AS-60431-1
$296.00 Excl. Tax
IMG-EN-AS-55576-1-01.jpg

MMP-9 (Catalytic Domain), human recombinant

Cat.Number : AS-55576-1
$119.00 Excl. Tax

Citations

Interrelationship between activation of matrix metalloproteinases and mitochondrial dysfunction in the development of diabetic retinopathy

Biochem Biophys Res Commun. . 2013 Sep 06 ; 438 760 | DOI : 10.1016/j.bbrc.2013.07.066

  • J.M. Santos

The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and differentiation

Genes Dev. . 2007 Nov 01 ; 21 2788 | DOI : 10.1101/gad.1544507

  • T. Okawa

HMGA1 Is a Determinant of Cellular Invasiveness and In vivo Metastatic Potential in Pancreatic Adenocarcinoma

Cancer Res . 2006 Dec 05 ; 66 11613 | DOI : 10.1158/0008-5472.CAN-06-1460

  • S-S. Liau

Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia inducible factor 1α, vascular endothelial growth factor A, and induction of thrombospondin-1 and TIMP3

J Biol Chem . 2012 Feb 17 ; 287 5492 | DOI : 10.1074/jbc.M111.283499

  • T. Neill

Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

Wound Repair Regen. . 2009 Nov 01 ; 17 832 | DOI : 10.1111/j.1524-475X.2009.00547.x

  • A. Moor

Inhibition of MMP-3 or -9 suppresses lipopolysaccharide-induced expression of proinflammatory cytokines and iNOS in microglia

JNC . 2008 Jul 04 ; 106(2) 770 | DOI : https://doi.org/10.1111/j.1471-4159.2008.05430.x

  • M. Woo

Homocysteine-Enhanced Proteolytic and Fibrinolytic Processes in Thin Intraluminal Thrombus and Adjacent Wall of Abdominal Aortic Aneurysm: Study In Vitro.

Biomed Res Int . 2018 Dec 12 ; 2018 3205324 | DOI : https://doi.org/10.1155/2018/3205324

  • A. Siennicka
  • et al

The Effects of Propofol on a Human in vitro Blood-Brain Barrier Model

Front Cell Neurosci. . 2022 May 11 ; 16 835649 | DOI : 10.3389/fncel.2022.835649

  • JM. Hughes
  • et al

Stepwise candidate drug screening for myopia control by using zebrafish, mouse, and Golden Syrian Hamster myopia models.

EBioMedicine . 2021 Mar 01 ; 65 103263 | DOI : https://doi.org/10.1016/j.ebiom.2021.103263

  • M-Y. Lin
  • et al