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Antibodies  >  Primary Antibodies  >  Cytoskeleton  >>  Anti-Actin antibody, alpha smooth muscle, polyclonal

Product Name Anti - Actin antibody, alpha smooth muscle, polyclonal
Size 50 µg
Catalog # 29553
US$ $193
Description

Host: Rabbit Species Reactivity: Human, mouseApplication: WB, IHCProduct Description: This polyclonal anti-actin antibody is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl of phosphate buffered saline (pH 7.4) containing 0.02% sodium azide. Immunogen: Rabbit polyclonal anti-Actin antibody was raised against a synthetic peptide corresponding to N-terminus of actin from human smooth muscle.Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. WB: 0.5 - 2 µg/mlIHC:1:50-1:200 for 10 minFigure 1. Western blot of actin, smooth muscle in 3T3 cell (Lane A), and mouse heart whole tissue lysate (Lane B) labeled with anti-Actin antibody alpha, smooth muscle (cat#29553) at a concentration of 1 ug/ml. Goat anti-rabbit IgG (H+L), highly cross-adsorbed, HiLyte™ Fluor750-labeled secondary antibody (Cat# 61056-05-H750 or 61056-1-H750) was used and the signals detected using an infrared-imaging system (Odyssey, Li-Cor). Figure 2. Human Leiomyoma stained with anti-Actin antibody (Cat# 29553) by IHC Histain Kit-AEC. Formalin-fixed tissues boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min, and probed at a dilution of 1:100 for 10 min at room temperature.

Images Anti-Actin antibody, alpha smooth muscle, <i>polyclonal</i>
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage 4°C
Product Citations Namavari, A. et al. (2012). Cyclosporine immunomodulation retards regeneration of surgically transected corneal nerves. Invest Ophthalmol Vis Sci 53, 732. doi: 10.1167/iovs.11-8445.
Chen, W. et al. (2011). Role of hepatic stellate cells on graft injury after small-for-size liver transplantation. J Gastro Hepatol 26, 1659. doi: 10.1111/j.1440-1746.2011.06781.x.
Lewandowski, R. et al. (2008). RXP-E: A connexin43-binding peptide that prevents action potential propagation block. Circ Res 103, 519.
Jay, PY. et al. (2007). Impaired mesenchymal cell function in Gata4 mutant mice leads to diaphragmatic hernias and primary lung defects. Dev Biol 301, 602. doi: http://dx.doi.org/10.1016/j.bbr.2011.03.031.
     
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