AnaSpec offers two types of fluoresceinated collagen (type I). This product is water soluble and is used for quick fluorometric measurement of collagenase activity. In this protease substrate, collagen is heavily labeled with FITC, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. This fluoresceinated collagen is useful for detecting MMP-1 activity.
Ref: Sela MN, et al. (2003). Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clin Oral Implants Res 14, 263-8; Baici A, et al. (1980). A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 108, 230-2; Sugiyama K, et al. (1980). A simple and rapid assay for collagenase activity using fluorescence-labeled substrate.Kurume Med J 27, 63-9.