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Assay Kits  >  Generic Protease Assay Kits  >>  SensoLyte® Green Protease Assay Kit *Fluorimetric*

Product Name SensoLyte® Green Protease Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 71124
US$ $247
Description

The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit contains: • Fluorescent labeled casein with high ratio of dye/protein (pH-insensitive) • Trypsin (as positive control) • Assay buffer • A detailed protocol

Kit Size: 500 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
Product Citations Kao, W-T. et al. (2012). Antioxidant and trypsin inhibition activity of dioscorin influenced by yam cultivars and eluent pH. Intl J Food Sci Tech 47, 1384. doi: 10.1111/j.1365-2621.2012.02984.x
Wei, Y. et al. (2009). Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases. Bioorganic and Medicinal Chem 17, 3003.
Groll, M. et al. (2008). A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism. Nature 452, 755.
Phuong, D. et al. (2008). Inhibitory effects of antrodins A–E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother Res 23, 582.
     
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