IMG-EN-AS-71103-01.jpg
Assay-kits

SensoLyte® MG Phosphate Assay Kit Colorimetric - 1 kit

$364.00
Check your price
  • Cat.Number : AS-71103
  • Availability :
    In stock

Quantity

Add to wishlist Bulk Quote

The MG (Malachite Green) Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate, and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes, or multi-well plates. The assays can be conveniently performed in 96 or 384-well plates for high-throughput screening of enzyme inhibitors. The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase, etc.) and phytase. The kit has the following components: - Highly purified Malachite Green reagent - Protein phosphatase inhibitor - Assay buffer - A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments with Z' factors of 0.7 to 0.9.

Specifications

Packaging
Kits components
  • Component A: 1 mM Phosphate, Standard: 1 mL Component B: MG Reagent: 20 mL
Properties
Absorbance (nm)
  • 600-660
Storage & stability
Storage Conditions
  • Store all components at room temperature.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

Downloads

You may also be interested in the following product(s)

IMG-EN-AS-72146-01.jpg

SensoLyte® pNPP Alkaline Phosphatase Assay Kit Colorimetric - 1 kit

Cat.Number : AS-72146
$232.00 Excl. Tax
IMG-EN-AS-71105-01.jpg

SensoLyte® pNPP Protein Phosphatase Assay Kit Colorimetric - 1 kit

Cat.Number : AS-71105
$324.00 Excl. Tax

Citations

Human acetyl-CoA carboxylase 2 expressed in silkworm Bombyx mori exhibits posttranslational biotinylation and phosphorylation

Appl Microbiol Biotech . 2014 Apr 17 ; 98 8201 | DOI : 10.1007/s00253-014-5715-6

  • I.W. Hwang

A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX

Biochem . 2014 Mar 26 ; 53 2126 | DOI : 10.1021/bi401597m

  • T. Lahiri

Interleukin-17A exacerbates ferric chloride-induced arterial thrombosis in rat carotid artery

Int J Inflammation . 2014 Apr 03 ; 247503 6 | DOI : http://dx.doi.org/10.1155/2014/247503

  • F. Maione

Impaired assembly and post-translational regulation of 26S proteasome in human end-stage heart failure

Circ Heart Fail . 2014 May 01 ; 6 | DOI : 10.1161/CIRCHEARTFAILURE.112.000119

  • S. Day

Hydrolysis of Extracellular ATP by Ectonucleoside Triphosphate Diphosphohydrolase (ENTPD) Establishes the Set Point for Fibrotic Activity of Cardiac Fibroblasts

JBC . 2013 May 15 ; 288 19040 | DOI : 10.1074/jbc.M113.466102

  • D. Lu
  • P.A. Insel

Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain

JBC . 2013 Jan 14 ; 288 7012 | DOI : 10.1074/jbc.M112.434209

  • K. Midde

The first prokaryotic trehalose synthase complex identified in the hyperthermophilic Crenarchaeon Thermoproteus tenax

PLoS ONE . 2013 Apr 23 ; | DOI : https://doi.org/10.1371/journal.pone.0061354

  • L.M. Zaparaty

Identification and characterization of a novel calcium-activated apyrase from Cryptosporidium parasites and its potential role in pathogenesis

PLoS ONE . 2012 Feb 07 ; | DOI : https://doi.org/10.1371/journal.pone.0031030

  • P.A. Manque

Optimization of a malachite green assay for detection of ATP hydrolysis by solubilized membrane proteins

Anal Biochem . 2012 Apr 13 ; 426 103 | DOI : 10.1016/j.ab.2012.04.016

  • B. Repen

Cancer Exosomes Express CD39 and CD73, Which Suppress T Cells through Adenosine Production

J Immunol . 2011 Jun 15 ; 187 676 | DOI : 10.4049/jimmunol.1003884

  • A. Clayton

NT5E mutations and arterial calcifications

N Engl J Med . 2011 Feb 03 ; 364 432 | DOI : 10.1056/NEJMoa0912923

  • C. Hilaire

Phenolics, phytic acid, and phytase in Canadian-grown low-tannin faba bean (Vicia faba L.) genotypes

J Agric Food Chem . 2011 Mar 10 ; 59 3763 | DOI : 10.1021/jf200338b

  • B.D. Oomah

Purification, primary structure, and properties of Euphorbia characias latex purple acid phosphatase

Biochemistry . 2011 Jun 01 ; 76 694 | DOI : 10.1134/S0006297911060101

  • F. Pinust

Characterization of LipL as a non-heme, Fe(II)-dependent α-ketoglutarate:UMP dioxygenase that generates uridine-5'-aldehyde during A-90289 biosynthesis.

J Biol Chem . 2011 Jan 07 ; 286 7885 | DOI : 10.1074/jbc.M110.203562

  • Z. Yang

Lyso-phosphatidylcholine induces osteogenic gene expression and phenotype in vascular smooth muscle cells

Atherosclerosis . 2010 Apr 14 ; 211 122 | DOI : 10.1016/j.atherosclerosis.2010.04.005

  • K. Vickers

Plakophilin 1 stimulates translation by promoting eIF4A1 activity

J Cell Biol . 2010 Feb 15 ; 188 463 | DOI : 10.1083/jcb.200908135

  • A. Wolf

Ecto-5′-nucleotidase (Nt5e/CD73)-mediated adenosine signaling attenuates TGFβ-2 induced elastin and cellular contraction

Am J Physiol Cell Physiol . 2023 Feb 01 ; 24(2) C327 | DOI : https://doi.org/10.1152/ajpcell.00054.2022

  • RA. Cuevas
  • et al

References

A Robotics-Based Automated Assay for Inorganic and Organic Phosphates

Anal Biochem . 1999 Jun 15 ; 271(1) 29 | DOI : https://doi.org/10.1006/abio.1999.4100

  • EB. Cogan
  • et al

A Sensitive, High-Volume, Colorimetric Assay for Protein Phosphatases

Pharm Res . 1994 May 01 ; 11(5) 759 | DOI : 10.1023/a:1018996817529

  • DK. Fisher
  • TJ. Higgins

Characterization of organic phosphorus in leachate from a grassland soil

Soil Biol Biochem . 2003 Oct 15 ; 35(10) 1317 | DOI : https://doi.org/10.1016/S0038-0717(03)00202-5

  • GS. Toor
  • et al