Labeling-detection

Fluorescein-5-maleimide - 25 mg

$221.00
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  • Cat.Number : AS-81405
  • Availability :
    In stock
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Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Fluorescein-5-maleimide is one of the most popular fluorescent dyes for thiol modifications of proteins.

Specifications

Chemistry
CAS registry number
  • 75350-46-8
Molecular Formula
  • C24H13NO7
Molecular Mass/ Weight
  • 427.4
Properties
Absorbance (nm)
  • 493
Emission (nm)
  • 515
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3.

elife . 2013 Aug 08 ; 2 e00828 | DOI : 10.7554/eLife.00828

  • H. Kamaduri

c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons.

Neurosci . 2007 Jun 20 ; 147(3) 680 | DOI : 10.1016/j.neuroscience.2007.05.009

  • J. Newbern

References

Chemical modifications of band 3 protein affect the adhesion of Plasmodium falciparum-infected erythrocytes to CD36

Mol Biochem Parasitol . 2004 Aug 01 ; 136(2) 243 | DOI : https://doi.org/10.1016/j.molbiopara.2004.04.005

  • E. Winograd
  • et al

SecY–SecY and SecY–SecG contacts revealed by site-specific crosslinking

FEBS Lett . 2002 Sep 11 ; 527(1-3) 159 | DOI : https://doi.org/10.1016/S0014-5793(02)03202-7

  • EO. Van Der Sluis
  • et al