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Labeling-detection

HiLyte™ Fluor 647 amine - 1 mg

$266.00
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  • Cat.Number : AS-81257
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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HiLyte™Fluor 647 amine is a carbonyl-reactive fluorescent labeling dye that generates the conjugates that are slightly red-shifted compared to those of Cy®5 dyes, resulting in an optimal match to filters designed for Cy®5 dye. Its conjugate may have better performance than Cy®5 for fluorescence polarization-based assays. Extinction Coefficient (M-1cm-1): 250,000 Fluorescence quantum yield: 0.33 Fluorescence Life Time (ns): 1.0

Specifications

Chemistry
Molecular Mass/ Weight
  • 1045.3
Properties
Absorbance (nm)
  • 649
Emission (nm)
  • 674
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • Water: Prepare 10 mg/ml dye stock solution in water. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Zeta Inhibitory Peptide Disrupts Electrostatic Interactions That Maintain Atypical Protein Kinase C in Its Active Conformation on the Scaffold p62.

J Biol Chem . 2015 Jul 17 ; 290(36) 21845 | DOI : 10.1074/jbc.M115.676221.

  • L.L. Tsai