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Labeling-detection

HiLyte™ Fluor 488 hydrazide - 1 mg

$217.00
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  • Cat.Number : AS-81163
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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HiLyte™ Fluor 488 hydrazide is a carbonyl-reactive fluorescent labeling dye. It can be used for labeling glycoproteins such as HRP. Fluorescence of HiLyte™ Fluor 488 fluorophore is independent of pH from 4 to 10. This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only above pH 10. Extinction Coefficient (M-1cm-1): 70,000; Fluorescence quantum yield: 0.91; Fluorescence Life Time (ns): 4.1

Specifications

Chemistry
Molecular Mass/ Weight
  • 388.4
Properties
Absorbance (nm)
  • 503
Emission (nm)
  • 528
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Longterm storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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