NBD-X acid is a building block that can be used to prepare peptide conjugates and other bioconjugates. It has higher conjugation efficiency than NBD-Cl and NBD-F. It should be noted that the NBD fluorescence is highly environment-sensitive.
McIntyre JC, et al. (1993). Synthesis and characterization of fluorescent neutral lipid analogs containing N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-aminohexanoic acid. Chem Phys Lipids 66, 171-80; Maruta S, et al. (2002). Interaction of a new fluorescent ATP analogue with skeletal muscle myosin subfragment-1. J Biochem (Tokyo) 131, 905-911.