IMG-EN-AS-25262-5-01.jpg
Peptides

Caspase 3 (Apopain) Substrate 1m, fluorogenic - 5 mg

$198.00
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  • Cat.Number : AS-25262-5
  • Availability :
    In production

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Caspase-3 substrate with Km = 9.7 µM; Caspase-7 substrate with Km = 11 µM;
AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DEVD-AMC
Sequence three letter code
  • Ac-Asp-Glu-Val-Asp-AMC
CAS registry number
  • 169332-61-0
Molecular Formula
  • C30H37N5O13
Molecular Mass/ Weight
  • 675.7
Properties
Absorbance (nm)
  • 350
Emission (nm)
  • 440
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Trophoblast differentiation modulates the activity of caspases in primary cultures of term human trophoblasts.

Ped Res . 2002 Sep 01 ; 52(3) 411 | DOI : 10.1203/00006450-200209000-00018

  • K. Yusef

Cholecystokinin induces caspase activation and mitochondrial dysfunction in pancreatic acinar cells: Roles in cell injury processes of pancreatitis.

J Biol Chem . 2002 Apr 18 ; 277(25) 22393 | DOI : 10.1074/jbc.M202929200

  • A.S. Gukovskaya

Induction of apoptosis by rosemary polyphenols in HL-60 cells.

Orient Foods Herbs chapter . 2003 Aug 05 ; 8 121 | DOI : 10.1021/bk-2003-0859.ch008.

  • S.Y. Lin-Shiau

Curcumin ameliorates ethanol and nonethanol experimental pancreatitis.

Am J Physiol Gastrointest Liver Physiol. . 2003 Jan 01 ; 284(1) G85 | DOI : 10.1152/ajpgi.00138.2002

  • I. Gukovsky

Differential regulation of the intrinsic pathway of apoptosis in brain and liver during ageing.

FEBS Lett . 2006 Jun 09 ; 580(15) 3739 | DOI : 10.1016/j.febslet.2006.05.066

  • V. Stoka

References

A combinatorial approach defines specificities of members of the caspase family and granzyme B.

J Biol Chem . 1997 Jul 01 ; 272(29) 17907 | DOI : https://doi.org/10.1074/jbc.272.29.17907

  • NA Thornberry

A combinatorial approach for determining protease specificities: application to interleukin-1 beta converting enzyme (ICE)

Chem Biol . 1997 Feb 07 ; 4(2) 149 | DOI : https://doi.org/10.1016/S1074-5521(97)90258-1

  • TA. Rano

Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization

Protein Expr Purif . 2002 May 25 ; 18(3) 378 | DOI : https://doi.org/10.1006/prep.2000.1205

  • KA. Koeplinger
  • et al

Ceramide induces apoptosis via CPP32 activation

FEBS Lett . 1996 Sep 13 ; 395(2-3) 267 | DOI : https://doi.org/10.1016/0014-5793(96)01050-2

  • N. Mizushima
  • et al

Inhibition of human caspases by peptide-based and macromolecular inhibitors

J Biol Chem . 1998 Dec 01 ; 273(49) 32608 | DOI : https://doi.org/10.1074/jbc.273.49.32608

  • M. Garcia-Calvo
  • et al

Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas

J Clin Invest . 1999 Jan 01 ; 103(1) 137 | DOI : https://doi.org/10.1172/JCI4765

  • WA. Faubion
  • et al

Substrate and inhibitor specificity of interleukin-1β-converting enzyme and related caspases.

J Biol Chem . 1997 Mar 01 ; 272(11) 7223 | DOI : https://doi.org/10.1074/jbc.272.11.7223

  • N. Margolin
  • et al