Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Stay Logged In

Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Fluorescent Dyes  >  Enzyme Detection Reagents  >>  FDG

Product Name FDG
[Fluorescein di - β - D - galactopyranoside]
Size 5 mg
Catalog # AS-85600
US$ $121

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.

Minimum Purity: 95%

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Yang NC and Hu ML (2004). A fluorimetric method using fluorescein di-?-D-galactopyranoside for quantifying the senescence-associated ?-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem 325, 337-43, Knapp T, et al. (2003). Detection of ?-lactamase reporter gene expression by flow cytometry. Cytometry 51A, 68-78, Nguyen DG and Hildreth JE (2003). Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages. Eur J Immunol 33, 483-93, Poot M and Arttamangkul S (1997). Verapamil inhibition of enzymatic product efflux leads to improved detection of ?-galactosidase activity in lacZ-transfected cells. Cytometry 28, 36-41, Chung JD, et al. (1995). Flow cytometric study of differentiating cultures of Bacillus subtilis. Cytometry 20, 324-33, Ikenaka K, et al. (1990). Reliable transient promoter assay using fluorescein-di-?-D-galactopyranoside substrate. DNA Cell Biol 9, 279-86.
Molecular Weight 656.59
Spectral Properties Abs/Em = 492/520 nm
Solvent System DMSO
Product Citations Tricoire, L. et al. (2010). Common Origins of Hippocampal Ivy and Nitric Oxide Synthase Expressing Neurogliaform Cells. J Neurosci 30, 2165.
  < Back