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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  FDG

Product Name FDG
[Fluorescein di - β - D - galactopyranoside]
Size 5 mg
Catalog # AS-85600
US$ $121
Purity Minimum Purity: 95%
Description

Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References 1. Yang NC and Hu ML (2004). Anal Biochem 325, 337-43,
2. Knapp T, et al. (2003). Cytometry 51A, 68-78,
3. Nguyen DG and Hildreth JE (2003). Eur J Immunol 33, 483-93,
4. Poot M and Arttamangkul S (1997). Cytometry 28, 36-41,
5. Chung JD, et al. (1995). Cytometry 20, 324-33,
6. Ikenaka K, et al. (1990). DNA Cell Biol 9, 279-86.
Molecular Weight 656.587
Molecular Formula C32H32O15
Spectral Properties Abs/Em = 492/520 nm
Solvent System DMSO
Product Citations Tricoire, L. et al. (2010). Common Origins of Hippocampal Ivy and Nitric Oxide Synthase Expressing Neurogliaform Cells. J Neurosci 30, 2165.
     
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