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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  Resorufin β-D-Galactopyranoside

Product Name Resorufin β - D - Galactopyranoside
Size 25 mg
Catalog # AS-85617
US$ $93
Purity Minimum Purity: 95%
Description

Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. This substrate is especially useful for sensitive enzyme measurements in ELISAs. The relatively low pKa (~6.0) of resorufin (the enzymatic hydrolysis product of resorufin galactoside) with Ex/Em=573/585 nm permits continuous measurement of enzymatic activity. Resorufin galactoside has also been used to quantitate β-galactosidase activity in single yeast cells by flow cytometry and to detect immobilized β-galactosidase activity.

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References 1. Yang NC and Hu ML (2004). Anal Biochem 325, 337-43.
2. Chiocconi A, et al. (2000). Carbohydr Res 323, 7-13.
3. Hakamata W, et al. (2000). Carbohydr Res 324, 107-15.
4. Weiss DJ, et al. (1997). Hum Gene Ther 8, 1545-54.
5. Lukomskaya IS, et al. (1996). Clin Chim Acta 244, 145-54.
6. Usui T, et al. (1992). Anal Biochem 202, 61-7.
7. Cousin MA, et al. (1989). J Appl Bacteriol 66, 311-7.
8. Bowen DM and Radin NS (1969). JNC 16, 457-460.
Molecular Weight 375.33
Molecular Formula C18H17NO8
Spectral Properties Abs/Em = 573/585 nm
Solvent System DMSO
     
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