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Assay-kits

SensoLyte® 520 MMP-2 Assay Kit Fluorimetric - 1 kit

$554.00
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  • Cat.Number : AS-71151
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target.
SensoLyte® 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-2, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-2 activity, please choose the SensoLyte® Plus MMP-2 Assay Kit, Cat# AS-72224.

Specifications

Packaging
Kits components
  • Component A: MMP-2 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 60 µL Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520: nm 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Organic mercury. Handle with care! Do not dispose into sink!): 1 M, 20 µL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Chemistry
UniProt number
  • P08253
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 15 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells

PLoS One . 2014 Apr 11 ; 9 e93576 | DOI : 10.1371/journal.pone.0093576

  • B. Liu

ARP101, a selective MMP-2 inhibitor, induces autophagy-associated cell death in cancer cells

Biochem Biophys Res Commun . 2011 Jan 28 ; 404 1039 | DOI : 10.1016/j.bbrc.2010.12.106

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Fluid shear stress regulates the invasive potential of glioma cells via modulation of migatory activity and matrix metalloproteinase expression

PLoS One . 2011 May 26 ; 6 e20348 | DOI : 10.1371/journal.pone.0020348

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A novel pharmacophore model for the design of anthrax lethal factor inhibitors

Chem Biol Drug Des . 2010 Sep 01 ; 76 263 | DOI : 10.1111/j.1747-0285.2010.01000.x

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Interstitial flow promotes vascular fibroblast, myofibroblast, and smooth muscle cell motility in 3-D collagen I via upregulation of MMP-1

Am J Physiol Heart Circ Physiol . 2009 May 22 ; 297 H1225 | DOI : 10.1152/ajpheart.00369.2009

  • Z-D. Shi

Quantification of pelvic organ prolapse in mice: Vaginal protease activity precedes increased MOPQ scores in fibulin 5 knockout mice

Biol Reprod . 2008 Nov 05 ; 80 407 | DOI : 10.1095/biolreprod.108.072900

  • C. Wieslander

A high-throughput screening approach to anthrax lethal factor inhibition

Bioorg Chem . 2007 Feb 22 ; 35 306 | DOI : 10.1016/j.bioorg.2006.12.005

  • S.L. Johnson

Regulation of elastolytic proteases in the mouse vagina during pregnancy, parturition, and puerperium

Biol Reprod . 2007 Nov 14 ; 78 521 | DOI : 10.1095/biolreprod.107.063024

  • C. Wieslander

Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament fibroblasts.

Am J Sports Med . 2014 Mar 13 ; 42(5) 1211 | DOI : 10.1177/0363546514524710

  • M.A. Pifer

Administration of anti-inflammatory M2 macrophages suppresses progression of angiotensin II-induced aortic aneurysm in mice.

Scientific Rep . 2023 Feb 25 ; 13 1380 | DOI : https://doi.org/10.1038/s41598-023-27412-x

  • S. Ashida
  • et al

Effects of Dietary Copper-Methionine on Matrix Metalloproteinase-2 in the Lungs of Cold-Stressed Broilers as an Animal Model for Pulmonary Hypertension

Biol Trace Elem Res . 2016 Jan 08 ; 172(2) 504 | DOI : 10.1007/s12011-015-0612-0

  • M. Bagheri Varzaneh
  • et al

Disruption of epithelial barrier by quorum-sensing N-3-(oxododecanoyl)-homoserine lactone is mediated by matrix metalloproteinases.

Am J Physiol Gastrointest Liver Physiol . 2014 Jun 01 ; 306(11) G992 | DOI : 10.1152/ajpgi.00016.2014

  • S. Eum
  • et al