IMG-EN-AS-71153-01.jpg
Assay-kits

SensoLyte® 520 MMP-7 Assay Kit Fluorimetric - 1 kit

$554.00
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  • Cat.Number : AS-71153
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MMP-7 (matrilysin, PUMP) is involved in biological events, such as tissue remodeling, cell proliferation and host defenses.
SensoLyte® 520 MMP-7 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-7, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: MMP-7 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 60 µL Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Organic mercury. Handle with care! Do not dispose into sink!): 1 M, 20 µL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Chemistry
UniProt number
  • P09237
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 10 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Sphingosine-1-phosphate induced epithelial-mesenchymal transition of hepatocellular carcinoma via an MMP-7/syndecan-1/TGF-β autocrine loop

Oncotarget. . 2016 Sep 27 ; 7(39) 63324 | DOI : 10.18632/oncotarget.11450

  • Y. Zeng
  • et al

Usefulness of matrix metalloproteinase-7 in saliva as a diagnostic biomarker for laryngopharyngeal reflux disease.

Sci Rep . 2021 Aug 23 ; 11 17071 | DOI : https://doi.org/10.1038/s41598-021-96554-7

  • N-R. Im
  • et al

Pre-treatment With Ranibizumab Aggravates PDT Injury and Alleviates Inflammatory Response in Choroid-Retinal Endothelial Cells.

Front Cell Dev Biol . 2020 Jul 09 ; 8 608 | DOI : https://doi.org/10.3389/fcell.2020.00608

  • Y. Liu
  • et al