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Assay-kits

SensoLyte® 520 MMP-8 Assay Kit Fluorimetric - 1 kit

$554.00
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  • Cat.Number : AS-71154
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-8 (netrophil collagnease) is proposed as a potential anti-cancer drug target.
SensoLyte® 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: MMP-8 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 60 µL Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 20 µL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Chemistry
UniProt number
  • P22894
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Neisseria meningitidis Induces Brain Microvascular Endothelial Cell Detachment from the Matrix and Cleavage of Occludin: A Role for MMP-8

PLoS Pathog . 2010 Apr 29 ; 6(4) e1000874 | DOI : 10.1371/journal.ppat.1000874

  • A. Schubert-Unkmeir

Real-time impedance analysis of host cell response to meningococcal infection

J Microbiol Methods. . 2010 Nov 13 ; 84(1) 101 | DOI : 10.1016/j.mimet.2010.11.004

  • H. Slanina

Anti-inflammatory and anti-oxidant mechanisms of an MMP-8 inhibitor in lipoteichoic acid-stimulated rat primary astrocytes: involvement of NF-κB, Nrf2, and PPAR-γ signaling pathways.

J Neuroinflam . 2018 Nov 23 ; 15 326 | DOI : https://doi.org/10.1186/s12974-018-1363-6

  • E-J. Lee
  • et al

Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse.

Mediators Inflamm . 2015 Nov 13 ; 2015 569512 | DOI : https://doi.org/10.1155/2015/569512

  • AK. Barton
  • et al